Alexa Fluor® 647 Mouse Anti-Human CD365 (TIM-1)

BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD365 (TIM-1)

Name: Alexa Fluor® 647 Mouse Anti-Human CD365 (TIM-1)
Brand: BD Pharmingen™
SKU: 563957

クローン 1D12

(RUO)

Flow cytometric analysis of CD365 (TIM-1) expressed on human 769-P cells. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152; Left Panel) and 769-P (Renal cell adenocarcinoma, ATCC CRL-1933; Right Panel) cell lines were stained with BD Horizon™ Fixable Viability Stain 450 (Cat. No. 562247). The cells were washed and then stained with either Alexa Fluor® 647 Mouse Anti-Human CD365 (TIM-1) antibody (Cat. No. 563957; solid histogram), or Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714; dashed line histograms). The fluorescence histograms were derived from gated events with the forward and side light-scatter and Fixable Viability Stain 450 characteristics (data not shown) of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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製品詳細

Brand: BD Pharmingen™

Alternative Name: CD365; TIM1; TIM; TIMD1; TIMD-1; HAVCR1; HAVCR-1; HAVCR; KIM1; KIM-1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human TIM-1 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: X 10-67

RRID: AB_2738509

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
An isotype control should be used at the same concentration as the antibody of interest.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Fixable Viability Stain 450 RUO

サイズ 0.1 mg カタログ番号 562247

Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO

サイズ 100 Tests カタログ番号 557714

563957 Rev. 2

抗体の詳細

1D12

The 1D12 monoclonal antibody specifically binds to CD365, the T-cell immunoglobulin mucin receptor 1 (TIM-1). TIM-1 is expressed on kidney epithelial cells, T cells, and some hematopoietic and non-hematopoietic cells. CD365 (TIM-1) is a type 1 transmembrane glycoprotein that serves as a receptor for hepatitis A virus and is encoded by the HAVCR1 (Hepatitis A virus cellular receptor 1) gene. TIM-1 also serves as a receptor for phosphatidylserine which is exposed on the surface of apoptotic cells. TIM-1 can reportedly mediate the uptake of apoptotic cells through the recognition of phosphatidylserine and thus help maintain tissue homeostasis and self-tolerance. TIM-1 is likewise known as Kidney injury molecule 1 (KIM-1). It is highly expressed by cancerous kidneys, and upregulated in the proximal tubular epithelium and shed into the urine during acute and chronic kidney injury. CD365 (TIM-1) also functions as a costimulatory molecule for immune cells. It is expressed by activated CD4+ T cells and regulates the effector functions (eg, enhanced cytokine production) and survival of differentiated T cells, including those mediating Th2-like immune responses. Other ligands have been described for TIM-1 including TIM-4 and LMIR5 (also known as CD300b) which are expressed by myeloid cells. With respect to disease associations, the HAVCR1 gene has been linked to asthma, allergy, and some autoimmune diseases.

563957 Rev. 2

フォーマットの詳細

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: Alexa Fluor™ 647

励起源: Red 627-640 nm

最大励起波長: 653 nm

最大蛍光波長: 669 nm

563957 Rev.2

引用＆参考文献

Development References (7)

Binne LL, Scott ML, Rennert PD. Human TIM-1 associates with the TCR complex and up-regulates T cell activation signals. J Immunol. 2007; 178(7):4342-4350. (Biology). View Reference
Freeman GJ, Casasnovas JM, Umetsu DT, DeKruyff RH. TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.. Immunol Rev. 2010; 235(1):172-89. (Biology). View Reference
Kim HY, Eyheramonho MB, Pichavant M, et al. A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans. J Clin Invest. 2011; 121(3):1111-1118. (Clone-specific: Blocking, Functional assay). View Reference
Kobayashi N, Karisola P, Pena-Cruz V, et al. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity. 2007; 27(6):927-940. (Immunogen: Blocking, Flow cytometry, Functional assay). View Reference
Manangeeswaran M, Jacques J, Tami C, et al. Binding of hepatitis A virus to its cellular receptor 1 inhibits T-regulatory cell functions in humans. Gastroenterology. 2012; 142(7):1516-1525. (Clone-specific: Blocking, Functional assay). View Reference
Rennert PD. Novel roles for TIM-1 in immunity and infection. Immunol Lett. 2011; 141(1):28-35. (Clone-specific: Blocking). View Reference
Rodriguez-Manzanet R, DeKruyff R, Kuchroo VK, Umetsu DT. The costimulatory role of TIM molecules. Immunol Rev. 2009; 229(1):259-270. (Biology). View Reference

すべて表示する (7)

563957 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.