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BV510 Rat Anti-Mouse CD138
BV510 Rat Anti-Mouse CD138
Two-color flow cytometric analysis of CD138 expression on mouse bone marrow B lymphocytes. Bone marrow cells from C57BL/6 mice were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089/561878) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952, Left Panel) or with BD Horizon™ BV510 Rat Anti-Mouse CD138 antibody (Cat. No. 563192, Right Panel). Two-color flow cytometric dot plots showing the expression of CD138 (or Ig isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD138 expression on mouse bone marrow B lymphocytes. Bone marrow cells from C57BL/6 mice were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089/561878) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952, Left Panel) or with BD Horizon™ BV510 Rat Anti-Mouse CD138 antibody (Cat. No. 563192, Right Panel). Two-color flow cytometric dot plots showing the expression of CD138 (or Ig isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
製品詳細
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BD Horizon™
SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
NAMRU mouse mammary gland epithelial cell line NMuMG
Flow cytometry (Routinely Tested)
0.2 mg/ml
20969
AB_2738059
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Brilliant Violet™ 510 is a trademark of Sirigen.
563192 Rev. 1
抗体の詳細
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281-2

The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules.  Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization.  It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow, and antibody-secreting cells including plasma cells (but not mature peripheral B cells).  It is thus implicated in mediating B cell-matrix interactions.  CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational  modifications.  The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein.  Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

563192 Rev. 1
フォーマットの詳細
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BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV510
Violet 405 nm
327 nm, 405 nm
512 nm
563192 Rev.1
引用&参考文献
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Development References (10)

  1. Bernfield M, Kokenyesi R, Kato M, et al. Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans. Annu Rev Cell Biol. 1992; 8:365-393. (Biology). View Reference
  2. Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence). View Reference
  3. Fitzgerald ML, Wang Z, Park PW, Murphy G, Bernfield M. Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase. J Cell Biol. 2000; 148(4):811-824. (Clone-specific: Dot Blot, Fluorescence microscopy, Immunofluorescence). View Reference
  4. Hayashi K, Hayashi M, Jalkanen M, Firestone JH, Trelstad RL, Bernfield M. Immunocytochemistry of cell surface heparan sulfate proteoglycan in mouse tissues. A light and electron microscopic study. J Histochem Cytochem. 1987; 35(10):1079-1088. (Clone-specific: Immunohistochemistry). View Reference
  5. Jalkanen M, Nguyen H, Rapraeger A, Kurn N, Bernfield M. Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody. J Cell Biol. 1985; 101(3):976-984. (Immunogen: Dot Blot, ELISA, Fluorescence microscopy, Immunofluorescence, Radioimmunoassay, Western blot). View Reference
  6. Lalor PA, Nossal GJ, Sanderson RD, McHeyzer-Williams MG. Functional and molecular characterization of single, (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific, IgG1+ B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP. Eur J Immunol. 1992; 22(11):3001-3011. (Biology: Western blot). View Reference
  7. Sanderson RD, Lalor P, Bernfield M. B lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul. 1989; 1(1):27-35. (Clone-specific: Flow cytometry, Immunoaffinity chromatography, Immunohistochemistry, Western blot). View Reference
  8. Sanderson RD, Sneed TB, Young LA, Sullivan GL, Lander AD. Adhesion of B lymphoid (MPC-11) cells to type I collagen is mediated by integral membrane proteoglycan, syndecan. J Immunol. 1992; 148(12):3902-3911. (Clone-specific: Immunoaffinity chromatography, Radioimmunoassay). View Reference
  9. Saunders S, Jalkanen M, O'Farrell S, Bernfield M. Molecular cloning of syndecan, an integral membrane proteoglycan. J Cell Biol. 1989; 108(4):1547-1556. (Clone-specific). View Reference
  10. Wehrli N, Legler DF, Finke D. Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes. Eur J Immunol. 2001; 31(2):609-616. (Biology: Immunohistochemistry). View Reference
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563192 Rev. 1

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