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      BV421 Hamster Anti-Mouse CD28
      BV421 Hamster Anti-Mouse CD28
      Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes.  Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047/561835) and FITC Rat Anti-Mouse CD8 (Cat. No. 553030/553031/561966) antibodies and with either BD Horizon™ BV421 Armenian Hamster IgG2, κ Isotype Control (Cat. No. 562629; Left Panel) or BD Horizon™ BV421 Hamster Anti-Mouse CD28 antibody (Cat. No. 562764; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD28 (or Ig Isotype Control staining) versus CD4 and CD8 for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Hamster Anti-Mouse CD28
      Multicolor flow cytometric analysis of CD28 expression on C57BL/6 mouse splenocytes.  Splenic leucocytes were pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained simultaneously with FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047/561835) and FITC Rat Anti-Mouse CD8 (Cat. No. 553030/553031/561966) antibodies and with either BD Horizon™ BV421 Armenian Hamster IgG2, κ Isotype Control (Cat. No. 562629; Left Panel) or BD Horizon™ BV421 Hamster Anti-Mouse CD28 antibody (Cat. No. 562764; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD28 (or Ig Isotype Control staining) versus CD4 and CD8 for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      製品詳細
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      BD Horizon™
      Cd28; CD28 antigen; T-cell-specific surface glycoprotein CD28
      Mouse (QC Testing)
      Syrian Hamster IgG2, λ1
      Mouse EL-4 (T-cell lymphoma) Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12487
      AB_2737778
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
      4. Brilliant Violet™ 421 is a trademark of Sirigen.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      関連製品

      Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
      サイズ 0.1 mg カタログ番号 553141
      sampleImage/
      Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
      サイズ 0.5 mg カタログ番号 553142
      sampleImage/
      Stain Buffer (FBS) RUO
      サイズ 500 mL カタログ番号 554656
      sampleImage/
      Lysing Buffer RUO
      サイズ 100 mL カタログ番号 555899
      sampleImage/
      BV421 Hamster IgG2, Lambda Isotype Control RUO
      サイズ 50 µg カタログ番号 562629
      sampleImage/
      FITC Rat Anti-Mouse CD4 RUO
      サイズ 0.1 mg カタログ番号 553046
      sampleImage/
      詳細を表示
      562764 Rev. 2
      抗体の詳細
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      37.51

      The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes,  has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

      562764 Rev. 2
      フォーマットの詳細
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      562764 Rev.2
      引用＆参考文献
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      Development References (16)

      1. Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology: Apoptosis). View Reference
      2. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity. 1997; 6(3):245-255. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
      3. Gelfanov V, Lai YG, Gelfanova V, Dong JY, Su JP, Liao NS. Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells. J Immunol. 1995; 155(1):76-82. (Clone-specific: (Co)-stimulation, Flow cytometry, Stimulation). View Reference
      4. Gross JA, Callas E, Allison JP. Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992; 149(2):380-388. (Immunogen: (Co)-stimulation, Flow cytometry, Immunoprecipitation, Stimulation). View Reference
      5. Harding FA, Allison JP. CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help. J Exp Med. 1993; 177(6):1791-1796. (Clone-specific: (Co)-stimulation, Inhibition, Stimulation). View Reference
      6. Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature. 1992; 356(6370):607-609. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
      7. June CH, Bluestone JA, Nadler LM, Thompson CB. The B7 and CD28 receptor families. Immunol Today. 1994; 15(7):321-331. (Biology). View Reference
      8. Krummel MF, Allison JP. CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J Exp Med. 1995; 182(2):459-465. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
      9. Lepesant H, Pierres M, Naquet P. Deficient antigen presentation by thymic epithelial cells reveals differential induction of T cell clone effector functions by CD28-mediated costimulation. Cell Immunol. 1995; 161(2):279-287. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
      10. Marietta EV, Weis JJ, Weis JH. CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi. J Immunol. 1997; 159(6):2840-2848. (Clone-specific: (Co)-stimulation, Flow cytometry, Stimulation). View Reference
      11. Nandi D, Gross JA, Allison JP. CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells. J Immunol. 1994; 152(7):3361-3369. (Clone-specific: (Co)-stimulation, Flow cytometry, Stimulation). View Reference
      12. Nishio M, Spielman J, Lee RK, Nelson DL, Podack ER. CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis. J Immunol. 1996; 157(10):4347-4353. (Biology). View Reference
      13. Ong CJ, Lim AS, Teh HS. CD28-induced cytokine production and proliferation by thymocytes are differentially regulated by the p59fyn tyrosine kinase. J Immunol. 1997; 159(5):2169-2176. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
      14. Rakasz E, Hagen M, Sandor M, Lynch RG. Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules. Int Immunol. 1997; 9(1):161-167. (Clone-specific: Flow cytometry). View Reference
      15. Shahinian A, Pfeffer K, Lee KP, et al. Differential T cell costimulatory requirements in CD28-deficient mice. Science. 1993; 261(5121):609-612. (Biology). View Reference
      16. Wells AD, Gudmundsdottir H, Turka LA. Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. J Clin Invest. 1997; 100(12):3173-3183. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
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      562764 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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