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Purified Mouse anti-Bcl-6
Purified Mouse anti-Bcl-6
Analysis of Bcl-6 expression by Western blot and Immunohistochemistry.      Left Panel: Western blot analysis of Bcl-6 expressed by Human Jurkat (Cat. No. 611451) and Ramos cell lines. Cell lysates from untreated Jurkat (lanes 1-5) and Ramos (lanes 6-10) cells (15 µg total cellular protein/lane) were electrophoresed (SDS-PAGE), transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody (Clone K112-91; Cat. No. 561520) at concentrations of 2 (lanes 1, 6), 0.667 (lanes 2, 7), 0.222 (lanes 3, 8), 0.074 (lanes 4, 9) and 0.025 (lanes 5, 10) µg/ml. Bcl-6 is identified as a band of ~87-98  kDa in the Ramos cell lysate.      Middle Panel: Cell lysates from untreated Mouse BA/F3 (lanes 1-5) and A20 (lanes 6-10) cells (15 µg total cellular protein/lane) were electrophoresed (SDS-PAGE), transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 (Clone K112-91; Cat. No. 561520) antibody at concentrations of 2 (lanes 1, 6), 0.667 (lanes 2, 7), 0.222 (lanes 3, 8), 0.074 (lanes 4, 9), and 0.025 (lanes 5, 10) µg/ml. Bcl-6 is identified as a band of ~87-98 kDa in the A20 cell lysate.      Right Panel: Bcl-6 staining of human tonsil. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; Left Image) or Purified Mouse Anti-Bcl-6 antibody (Clone K112-91; Cat. No. 561520; Middle and Right Images), with Hematoxylin counterstaining. Bcl-6 is detected in the nuclei of the lymphocytes within the lymphoid follicles of the tonsil. Original magnifications: 20X and 40X as shown.
Analysis of Bcl-6 expression by Western blot and Immunohistochemistry.      Left Panel: Western blot analysis of Bcl-6 expressed by Human Jurkat (Cat. No. 611451) and Ramos cell lines. Cell lysates from untreated Jurkat (lanes 1-5) and Ramos (lanes 6-10) cells (15 µg total cellular protein/lane) were electrophoresed (SDS-PAGE), transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody (Clone K112-91; Cat. No. 561520) at concentrations of 2 (lanes 1, 6), 0.667 (lanes 2, 7), 0.222 (lanes 3, 8), 0.074 (lanes 4, 9) and 0.025 (lanes 5, 10) µg/ml. Bcl-6 is identified as a band of ~87-98  kDa in the Ramos cell lysate.      Middle Panel: Cell lysates from untreated Mouse BA/F3 (lanes 1-5) and A20 (lanes 6-10) cells (15 µg total cellular protein/lane) were electrophoresed (SDS-PAGE), transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 (Clone K112-91; Cat. No. 561520) antibody at concentrations of 2 (lanes 1, 6), 0.667 (lanes 2, 7), 0.222 (lanes 3, 8), 0.074 (lanes 4, 9), and 0.025 (lanes 5, 10) µg/ml. Bcl-6 is identified as a band of ~87-98 kDa in the A20 cell lysate.      Right Panel: Bcl-6 staining of human tonsil. Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; Left Image) or Purified Mouse Anti-Bcl-6 antibody (Clone K112-91; Cat. No. 561520; Middle and Right Images), with Hematoxylin counterstaining. Bcl-6 is detected in the nuclei of the lymphocytes within the lymphoid follicles of the tonsil. Original magnifications: 20X and 40X as shown.
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BD Pharmingen™
BCL6; B-cell lymphoma 6 protein; LAZ3; Laz-3, ZBTB27, ZNF51
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Human Bcl-6 Recombinant Protein
Western blot (Routinely Tested), Immunohistochemistry-paraffin, Intracellular staining (flow cytometry) (Tested During Development)
87–98 kDa
0.5 mg/ml
604, 12053
AB_10713172
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This product is sold under license to the following patent: US Patent No. 6,174,997.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561520 Rev. 4
抗体の詳細
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K112-91

The K112-91 monoclonal antibody specifically binds to Bcl-6. Bcl-6 was first identified as a proto-oncogene frequently deregulated by chromosomal translocations in non-Hodgkin B-cell lymphomas. It is a nuclear transcriptional repressor of the BTB/POZ zinc-finger family of transcription factors. In addition to its roles in cancer, Bcl-6 plays important roles in the differentiation of normal cells including B cells, thymocytes, CD4+ or CD8+ T cells. Bcl-6 is highly expressed in germinal center B cells, where it promotes the germinal center reaction by inducing proliferation and inhibiting the DNA-damage response. Bcl-6 has been identified as a key factor in promoting the differentiation of CD4+ follicular T helper (Tfh) cells that are involved in promoting germinal center formation and providing help to B cells. The interplay of Bcl-6 and another transcriptional repressor, Blimp-1, is thought to be critical in defining the results of both B-cell and T-cell differentiation.

561520 Rev. 4
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
561520 Rev.4
引用&参考文献
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Development References (9)

  1. Baumjohann D, Okada T, Ansel KM. Cutting Edge: Distinct Waves of BCL6 Expression during T Follicular Helper Cell Development. J Immunol. 2011; 187(5):2089-2092. (Clone-specific: Flow cytometry). View Reference
  2. Choi YS, Kageyama R, Eto D, et al. ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6.. Immunity. 2011; 34(6):932-46. (Clone-specific). View Reference
  3. Crotty S, Johnston RJ, Schoenberger SP. Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation. Nat Immunol. 2010; 11(2):114-120. (Biology). View Reference
  4. Crotty S. Follicular Helper CD4 T Cells (Tfh). Annu Rev Immunol. 2011; 29(1):621-663. (Biology). View Reference
  5. Fazilleau N, McHeyzer-Williams LJ, Rosen H, McHeyzer-Williams MG. The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding. Nat Rev Immunol. 2009; 10(4):375-384. (Biology). View Reference
  6. Johnston RJ, Poholek AC, DiToro D, et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.. Science. 2009; 325(5943):1006-10. (Biology). View Reference
  7. Klein U, Dalla-Favera R. Germinal centres: role in B-cell physiology and malignancy. Nat Rev Immunol. 2008; 8(1):22-33. (Biology). View Reference
  8. Nurieva RI, Chung Y, Martinez GJ, et al. Bcl6 mediates the development of T follicular helper cells. Science. 2009; 325(5943):1001-1005. (Biology). View Reference
  9. Ye BH, Lista F, Lo Coco F, et al. Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma. Science. 1993; 262(5134):747-750. (Biology). View Reference
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561520 Rev. 4

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.