Preparation and Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Cy is a trademark of GE Healthcare.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
The 49-H4 antibody specifically binds to the ThB antigen (Ly-6D), a 15-kDa GPI-anchored protein of the Ly-6 Multigene Family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes (but not peripheral T lymphocytes). There is strain-to-strain variation in ThB antigen expression on splenic B cells. Strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti-ThB reagents than Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB); B cells of Ly-6.1 and Ly-6.2 hybrids stain with intermediate intensity. The proportion of ThB+ B cells to thymocytes do not differ significantly among strains.
Development References (6)
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Boyd RL, Tucek CL, Godfrey DI. The thymic microenvironment. Immunol Today. 1993; 14(9):445-459. (Biology). View Reference
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Eckhardt LA, Herzenberg LA. Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics. 1980; 11(3):275-291. (Immunogen: Cytotoxicity). View Reference
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Godfrey DI, Izon DJ, Tucek CL, Wilson TJ, Boyd RL. The phenotypic heterogeneity of mouse thymic stromal cells. Immunology. 1990; 70(1):66-74. (Biology). View Reference
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Gumley TP, McKenzie IF, Kozak CA, Sandrin MS. Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB). J Immunol. 1992; 149(8):2615-2618. (Biology). View Reference
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Gumley TP, McKenzie IF, Sandrin MS. Polymorphism at the mouse Thb locus. Immunogenetics. 1994; 39(6):390-394. (Clone-specific: Immunoprecipitation). View Reference
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Matossian-Rogers A, Rogers P, Ledbetter JA, Herzenberg LA. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics. 1982; 15(6):591-599. (Clone-specific: Immunoprecipitation). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
