Biotin Rat IgG2b, κ Isotype Control

BD Pharmingen™ Biotin Rat IgG2b, κ Isotype Control

Name: Biotin Rat IgG2b, κ Isotype Control
Brand: BD Pharmingen™
SKU: 555847

クローン R35-38

(RUO)

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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製品詳細

Brand: BD Pharmingen™

Isotype: Rat IgG2b, κ

Application: Flow cytometry, Isotype control (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_396170

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

関連製品

PE Streptavidin RUO

サイズ 0.5 mg カタログ番号 554061

555847 Rev. 5

抗体の詳細

R35-38

The R35-38 hybridoma produces monoclonal rat IgG2b, κ. The target molecule specificity of this clone is unknown. R35-38 has little or no reactivity with mammalian cells and has been found useful as an immunoglobulin isotype control.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555847 Rev. 5

フォーマットの詳細

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

フォーマット: Biotin

555847 Rev.5

引用＆参考文献

Development References (1)

Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference

555847 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.