製品詳細
BD Pharmingen™
Mouse (QC Testing)
Mouse BALB/c IgG2a, κ
Mouse C57BL/6 Splenocytes
Flow cytometry (Routinely Tested), Immunofluorescence, Immunoprecipitation (Reported)
0.5 mg/ml
14972
AB_394925
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Purified Mouse IgG2a κ Isotype Control RUO
サイズ
0.5 mg
カタログ番号
553454
FITC Goat Anti-Mouse IgG/IgM RUO
サイズ
0.5 mg
カタログ番号
555988
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Biotin Mouse Anti-Mouse H-2K[b] RUO
サイズ
1 mL
カタログ番号
550550
553567 Rev. 13
抗体の詳細
AF6-88.5
The AF6-88.5 antibody recognizes the H-2Kb MHC class I alloantigen. Reactivity with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v) has not been observed.
553567 Rev. 13
フォーマットの詳細
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553567 Rev.13
引用＆参考文献
Development References (3)
-
Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen). View Reference
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Pérarnau B, Saron MF, Reina San Martin B, et al. Single H2Kb, H2Db and double H2KbDb knockout mice: peripheral CD8+ T cell repertoire and anti-lymphocytic choriomeningitis virus cytolytic responses.. Eur J Immunol. 1999; 29(4):1243-52. (Clone-specific: Immunohistochemistry). View Reference
-
Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific: Immunoprecipitation). View Reference
553567 Rev. 13
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
