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      Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain
      製品詳細
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      BD Pharmingen™
      Igl; Ig λ; Immunoglobulin lambda chain complex; Ig λ1/λ2/λ3
      Mouse (QC Testing)
      Rat IgG2a, κ
      Pooled Mouse Ig
      ELISA (Routinely Tested)
      0.5 mg/ml
      111519
      AB_394853
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      推奨アッセイ手順

      For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain, cocktailed with Biotin Rat Anti-Mouse Ig, κ Light Chain, (Cat. No. 559750) is optimal for detection with any of the following anti-mouse Ig isotype-specific mAbs: Purified Rat Anti-Mouse IgG1, (Cat. No. 553440); Purified Rat Anti-Mouse IgG2a, (Cat. No. 553387); Purified Rat Anti-Mouse IgG2b, (Cat. No. 553392); R40-82 [available by custom order]; Purified Rat Anti-Mouse IgM, (Cat. No. 553435); Purified Rat Anti-Mouse IgA, (Cat. No. 556969); Purified Rat Anti-Mouse IgE, (Cat. No. 553413) for capture, respectively.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553433 Rev. 11
      抗体の詳細
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      R26-46

      The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.

      553433 Rev. 11
      フォーマットの詳細
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553433 Rev.11
      引用＆参考文献
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      Development References (2)

      1. Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. (Clone-specific). View Reference
      2. Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. (Clone-specific). View Reference
      553433 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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