PE Rat Anti-Mouse Vα 8.3 TCR

BD Pharmingen™ PE Rat Anti-Mouse Vα 8.3 TCR

Name: PE Rat Anti-Mouse Vα 8.3 TCR
Brand: BD Pharmingen™
SKU: 553377

クローン B21.14

(RUO)

Two-color analysis of the expression of Vα 8.3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated B21.14, FITC-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553046/553047), and FITC-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Spectrum Viewer

Protocol Library

Scientific Resources

製品詳細

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

Immunogen: Soluble αβ TCR from mouse cytotoxic T-cell clone CW3/1.1

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_394821

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

PE Rat IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 553925

FITC Rat Anti-Mouse CD4 RUO

サイズ 0.1 mg カタログ番号 553046

FITC Rat Anti-Mouse CD8a RUO

サイズ 0.1 mg カタログ番号 553030

553377 Rev. 10

抗体の詳細

B21.14

The B21.14 monoclonal antibody specifically recognizes some members of the Vα 8 T-cell Receptor (TCR) subfamily of mice having the a, b, c, and d haplotypes of the Tcra gene complex (e.g., all strains tested). It recognizes an epitope in the CDR1 of Vα 8.3, but not Vα 8.2, TCR subfamily member, as does the KT50 mAb (Cat. No. 557096, for the biotinylated antibody). Site-directed mutagenesis has identified three amino acids which are necessary for antibody reactivity and which are unique to Vα 8.3 among the five functional Vα 8 TCR subfamily members. On a common H-2K[k] background and using the KT50 mAb, the frequency of Vα 8.3 TCR-bearing T lymphocytes has been demonstrated to be higher in Tcra[a/a] mice than in Tcra[a/b] mice. Furthermore, studies of congenic strains suggest that CD8+ Vα 8.3 TCR-bearing T lymphocytes undergo negative selection in mice expressing MHC class I antigens of the H-2[d] haplotype.

553377 Rev. 10

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

553377 Rev.10

引用＆参考文献

Development References (4)

Brodnicki TC, Holman PO, Kranz DM. Reactivity and epitope mapping of single-chain T cell receptors with monoclonal antibodies. Mol Immunol. 1996; 33(3):253-263. (Biology). View Reference
Necker A, Rebai N, Matthes M, et al. Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells. Eur J Immunol. 1991; 21(12):3035-3040. (Immunogen). View Reference
Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
Tomonari K. Negative selection of Tcra-V8+CD8+ T cells by MHC class I molecules. Immunogenetics. 1992; 35(5):291-295. (Biology). View Reference

すべて表示する (4)

553377 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.