説明
Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death.
The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.
調製と保管
推奨アッセイ手順
In addition to Jurkat cell lysates, MOLT-4 (Human T-lymphoblasts; ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia; ATCC CCL-243), Hut-78 (Human T lymphoma; ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
| 説明 | 数量/容量 | Part Number | EntrezGene ID |
|---|---|---|---|
| Purified Mouse Anti- Bcl-x | 50 µg (3 ea) | 51-6646GR | N/A |
| Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
開発者向け参考資料 (4)
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Hsu YT, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A. 1997; 94(8):3668-3672. (Biology: Western blot). 参考文献を見る
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Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997; 272(21):13829-13834. (Immunogen: Western blot). 参考文献を見る
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Krajewski S, Krajewska M, Shabaik A, et al. Immunohistochemical analysis of in vivo patterns of Bcl-X expression. Cancer Res. 1994; 54(21):5501-5507. (Biology). 参考文献を見る
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Reed JC, Miyashita T, Krajewski S, et al. Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma. Cancer Treat Res. 1996; 84:31-72. (Biology). 参考文献を見る
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