Multiparameter flow cytometric analysis of Plexin C1 (CD232) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Plexin C1 (CD232) antibody (Cat. No. 566249; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of Plexin C1 (CD232) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Multiparameter flow cytometric analysis of Plexin C1 (CD232) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Plexin C1 (CD232) antibody (Cat. No. 566249; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of Plexin C1 (CD232) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
製品詳細
BD Pharmingen™
CD232; Plexin-C1; plexinC1; PLXC1; PLXN-C1; PLXNC1; VESPR
Human (QC Testing), Cow (Reported)
Mouse IgG2b, κ
Human Plexin C1 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739628
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
サイズ
50 µg
カタログ番号
565378
566249 Rev. 1
抗体の詳細
544232
The 544232 monoclonal antibody specifically recognizes human and mouse Plexin C1 (PLXNC1) which is also known as, Virus-encoded semaphorin protein receptor (VESPR), or CD232. Plexin C1 is a ~210 kDa single-pass type I membrane glycoprotein that belongs to the C subgroup of the plexin family of proteins. It is expressed on monocytes, dendritic cells, and neutrophils, as well as, some B lymphocytes and neuronal cells. Plexin C1 binds to Semaphorin 7A (CD108) and the viral semaphorin-like protein, A39R. Plexin C1 reportedly plays a role in cellular adhesion, migration, and phagocytosis.
566249 Rev. 1
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566249 Rev.1
引用＆参考文献
Development References (5)
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Comeau MR, Johnson R, DuBose RF, et al. A poxvirus-encoded semaphorin induces cytokine production from monocytes and binds to a novel cellular semaphorin receptor, VESPR.. Immunity. 1998; 8(4):473-82. (Biology). View Reference
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Kang S, Okuno T, Takegahara N, et al. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via αvβ1 integrin.. J Immunol. 2012; 188(3):1108-16. (Clone-specific: Flow cytometry, Functional assay). View Reference
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Liu H, Juo ZS, Shim AH, et al. Structural basis of semaphorin-plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1.. Cell. 2010; 142(5):749-61. (Biology). View Reference
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Myster F, Palmeira L, Sorel O, et al. Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.. J Virol. 2015; 89(7):3630-47. (Clone-specific: Flow cytometry). View Reference
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Spriggs M. CD232 (VESP-R) Summary and Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:513-514.
566249 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
