Alexa Fluor® 647 Mouse Anti-Human CD365 (TIM-1)
Clone 1D12 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD365; TIM1; TIM; TIMD1; TIMD-1; HAVCR1; HAVCR-1; HAVCR; KIM1; KIM-1
- Vol. Per Test 5 µl
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human TIM-1 Recombinant Protein
- Workshop No. X 10-67
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 1D12 monoclonal antibody specifically binds to CD365, the T-cell immunoglobulin mucin receptor 1 (TIM-1). TIM-1 is expressed on kidney epithelial cells, T cells, and some hematopoietic and non-hematopoietic cells. CD365 (TIM-1) is a type 1 transmembrane glycoprotein that serves as a receptor for hepatitis A virus and is encoded by the HAVCR1 (Hepatitis A virus cellular receptor 1) gene. TIM-1 also serves as a receptor for phosphatidylserine which is exposed on the surface of apoptotic cells. TIM-1 can reportedly mediate the uptake of apoptotic cells through the recognition of phosphatidylserine and thus help maintain tissue homeostasis and self-tolerance. TIM-1 is likewise known as Kidney injury molecule 1 (KIM-1). It is highly expressed by cancerous kidneys, and upregulated in the proximal tubular epithelium and shed into the urine during acute and chronic kidney injury. CD365 (TIM-1) also functions as a costimulatory molecule for immune cells. It is expressed by activated CD4+ T cells and regulates the effector functions (eg, enhanced cytokine production) and survival of differentiated T cells, including those mediating Th2-like immune responses. Other ligands have been described for TIM-1 including TIM-4 and LMIR5 (also known as CD300b) which are expressed by myeloid cells. With respect to disease associations, the HAVCR1 gene has been linked to asthma, allergy, and some autoimmune diseases.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.