Flow cytometric analysis of the expression of CD3e on spleen and thymus. LOU splenocytes were simultaneously stained with PE Mouse Anti-Rat CD4 (Cat. No. 554838; left panels), PE Mouse Anti-Rat CD8a (Cat. No. 559976/554857; left panels) and Purified Mouse Anti-Rat CD3 (Cat. No. 554830/550295/559974; bottom left panel) monoclonal antibodies, followed by FITC Rat Anti-Mouse IgG3 (Cat. No. 553403, left panels). Please note that Cd3- CD4+ monocytes and CD3- CD8a+ NK cells may be found in the rat spleen. LOU thymocytes were stained with Purified Mouse Anti-Rat CD3 (bottom right panel), followed by FITC Goat Anti-Mouse Ig (Cat. No. 554001, right panels). The fluorescence contour plots/histograms were derived from gated events based on the light-scattering characteristics of viable cells. .Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of the expression of CD3e on spleen and thymus. LOU splenocytes were simultaneously stained with PE Mouse Anti-Rat CD4 (Cat. No. 554838; left panels), PE Mouse Anti-Rat CD8a (Cat. No. 559976/554857; left panels) and Purified Mouse Anti-Rat CD3 (Cat. No. 554830/550295/559974; bottom left panel) monoclonal antibodies, followed by FITC Rat Anti-Mouse IgG3 (Cat. No. 553403, left panels). Please note that Cd3- CD4+ monocytes and CD3- CD8a+ NK cells may be found in the rat spleen. LOU thymocytes were stained with Purified Mouse Anti-Rat CD3 (bottom right panel), followed by FITC Goat Anti-Mouse Ig (Cat. No. 554001, right panels). The fluorescence contour plots/histograms were derived from gated events based on the light-scattering characteristics of viable cells. .Flow cytometry was performed on a BD FACScan™.
製品詳細
BD Pharmingen™
CD3 Complex; T3
Rat (QC Testing)
Mouse BALB/c IgG3, κ
PHA-stimulated rat lymph node and spleen cells
Flow cytometry (Routinely Tested), (Co)-stimulation, Cytotoxicity, Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation, Inhibition, Western blot (Reported)
0.5 mg/ml
AB_2073351
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
推奨アッセイ手順
For IHC, we recommend the use of Purified Mouse Anti-Rat CD3 (Cat. No. 550295) in our special formulation for immunohistochemistry .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Purified Mouse IgG3, κ Isotype Control RUO
サイズ
0.5 mg
カタログ番号
553486
PE Mouse Anti-Rat CD8a RUO
サイズ
0.2 mg
カタログ番号
554857
PE Mouse Anti-Rat CD4 RUO
サイズ
0.2 mg
カタログ番号
554838
PE Mouse Anti-Rat CD8a RUO
サイズ
0.1 mg
カタログ番号
559976
554830 Rev. 19
抗体の詳細
G4.18
The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.
554830 Rev. 19
フォーマットの詳細
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554830 Rev.19
引用＆参考文献
Development References (7)
-
Brenan M, Rees DJ. Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 1997; 27(11):3070-3079. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Morris DL, Komocsar WJ. Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats. J Pharmacol Toxicol Methods. 1997; 37(1):37-46. (Clone-specific). View Reference
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Naper C, Vaage JT, Lambracht D, et al. Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control. Eur J Immunol. 1995; 25(5):1249-1256. (Clone-specific: Cytotoxicity). View Reference
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Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry). View Reference
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Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Immunogen: (Co)-stimulation, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition, Stimulation). View Reference
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Strickland D, Kees UR, Holt PG. Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction. Immunology. 1996; 87(2):250-258. (Biology). View Reference
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Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology. 1995; 84(1):142-147. (Biology). View Reference
554830 Rev. 19
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
