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Perm/Wash Buffer I RUO

Perm/Wash Buffer I 

  • Brand BD Phosflow™
  • Application Intracellular staining (flow cytometry) (Routinely Tested) 
  • Storage Buffer Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

BD Phosflow™ Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins. BD Phosflow™ Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD Phosflow™ brand of intracellular phosphorylated signaling protein-specific antibodies. BD Phosflow™ Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.

Suggested Companion Products

Fix Buffer I RUO
250 mL Cat No: 557870

Lyse/Fix Buffer 5X RUO
250 mL Cat No: 558049

Fixation Buffer RUO
100 mL Cat No: 554655

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C.

Do not freeze.

Product Notices

  1. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


Flow cytometry: Dilute BD Phosflow™ Perm/Wash Buffer I 1:10 in distilled water prior to use. If desired, the diluted 1X BD™ Phosflow Perm/Wash Buffer I can be passed through a 0.45 µm filter to remove any residual precipitate. The BD Phosflow™ Perm/Wash Buffer I can be used to permeabilize or wash cells and to dilute antibodies for immunofluorescent staining of intracellular proteins.


  1. Assenmacher M, Schmitz J, Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24(5):1097-1101. View reference

  2. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. View reference

  3. Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. View reference

  4. Krutzik PO, Nolan GP. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A. 2003; 55(2):61-70. View reference

  5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. View reference

  6. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. View reference

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