Description
Tumor necrosis factor (TNF, formerly known as TNF-α), also known as cachectin, is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. This cytokine is a primary regulator of inflammatory responses. TNF is produced by macrophages, neutrophils, activated T and B lymphocytes, endothelial cells, smooth muscle cells, astrocytes, natural killer cells, lymphokineactivated killer cells, and some transformed cells. TNF, a 17.5 kD polypeptide of 157 amino acid residues, and LT-α (lymphotoxin alpha formerly known as TNF-α) are closely related (34% a.a. homology), bind to the same cellular receptors (CD120a and CD120b), and produce similar effects. TNFs play a critical role in the body's resistance to infection by inducing fever and activating macrophages and in the destruction of certain tumors. TNFs and Interleukin-1 function similarly. However, over-production of TNF has been associated with cytotoxic effects such as cachexia. The BD OptEIA™ ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance Characteristics “Recovery” and “Linearity” sections. The BD OptEIA Human TNF ELISA Kit II is for the quantitative determination of human TNF in serum, plasma, and cell culture supernatant.
Components Quantity Antibody Coated Wells 2 plates (12 strips x 8 wells) Detection Antibod 30 mL Standards 4 vials, lyophilized Enzyme Concentrate (250x 150 µL Standard/Sample Diluent 30 mL ELISA Diluent 12 mL Wash Concentrate (20x) 100 mL TMB One-Step Substrate Reagent 30 mL Stop Solution 13 mL Plate Sealers 4 sheets
Preparation and Storage
1. Store kit at 2 – 8°C. Do not use kit after expiration date. 2. Before use, bring all reagents to room temperature (18 – 25°C). Immediately after use, return to proper storage conditions. 3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 – 8°C).
