FITC Mouse Anti-Human CD44
Clone L178 (RUO (GMP))
- Concentration 12.5 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human TCR γδ+ Thymocytes
- Workshop No. V NK14, S329
- Entrez Gene ID 960
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The CD44 antibody, clone L178, is derived from hybridization of Sp2/0 myeloma mouse cells with spleen cells from BALB/c mice immunized with TCR-γ/δ+ thymocytes.
The CD44 (Leu-44) antibody recognizes a cell-surface glycoprotein that exists in a variety of isoforms. It is synthesized as a molecule with a molecular weight of 37 kilodaltons (kDa). Upon glycosylation, it is converted to an 80- to 95-kDa form. Alternatively, a 180- to 200-kDa form results from the addition of chondroitin sulfate.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline — Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.