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FITC Mouse Anti-Human CD44 L178 RUO (GMP)

FITC Mouse Anti-Human CD44 

Clone  L178   (RUO (GMP))

  • Concentration 12.5 μg/mL
  • Vol. Per Test 20 μL
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Human TCR γδ+ Thymocytes
  • Workshop No. V NK14, S329
  • Entrez Gene ID 960 
  • Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
  • Regulatory Status RUO (GMP)
Product Details Recommended Assay References

Description

The CD44 antibody, clone L178, is derived from hybridization of Sp2/0 myeloma mouse cells with spleen cells from BALB/c mice immunized with TCR-γ/δ+ thymocytes.

The CD44 (Leu-44) antibody recognizes a cell-surface glycoprotein that exists in a variety of isoforms. It is synthesized as a molecule with a molecular weight of 37 kilodaltons (kDa). Upon glycosylation, it is converted to an 80- to 95-kDa form. Alternatively, a 180- to 200-kDa form results from the addition of chondroitin sulfate.

Format
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.


  1. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline — Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.

  2. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

  3. Aruffo A, Stamenkovic I, Melnick M, Unterhill CB, Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 1990;61:1303-1313.

  4. Coombe DR, Rider CC. Lymphocyte homing receptors cloned: A role for anionic polysaccharides in lymphocyte adhesion. Immunol Today. 1989;10:289.

  5. Dalchau R. Chemical composition and tissue distribution of the human CD44 glycoprotein. In: Knapp W, Dörken W, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:622-625.

  6. Gallatin WM, Gale MJ Jr, Hoffman PA, et al. Selective replication of simian immunodeficiency virus in a subset of CD4+ lymphocytes. Proc Natl Acad Sci USA. 1989;86:3301-3305.

  7. Haynes BF, Telen MJ, Hale LP, Denning SM. CD44 – a molecule involved in leukocyte adherence and T-cell activation. Immunol Today. 1989;10:423-428.

  8. Picker LJ, de los Toyos J, Telen MJ, Haynes BF, Butcher EC. Monoclonal antibodies against the CD44 (In-[Lu]-related p80) and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J Immunol. 1989;142:2046-2051.

  9. Shimizu Y, Van Seventer GA, Siraganian R, Wahl L, Shaw S. Dual role of the CD44 molecule in T-cell adhesion and activation. J Immunol. 1989;143:2457-2463.

  10. St. John T, Meyer J, Idzerda R, Gallatin WM. Expression of CD44 confers a new adhesive phenotype on transfected cells. Cell. 1990;60:45-52.

  11. Stoll M, Dalchau R, Schmidt RE. Cluster report: CD44. In: Knapp W, Dörken W, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:619-622

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