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Purified Mouse Anti-Stat5 89/Stat5 RUO

Purified Mouse Anti-Stat5 

Clone  89/Stat5   (RUO)

  • Brand BD Transduction Laboratories™
  • Alternative Name MGF
  • Concentration 250 µg/ml
  • Isotype Mouse IgG2b
  • Reactivity Human (QC Testing)  Mouse, Rat, Dog, Rabbit (Tested in Development) 
  • Application Western blot (Routinely Tested) 
    Immunofluorescence, Immunohistochemistry (Tested During Development) 
    Immunoprecipitation (Reported) 
  • Immunogen Sheep Stat5 aa. 451-649
  • Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

Stat5, also known as mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology has led to its classification as a member of the Stat family. The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation.

Format
  • Format Purified
Suggested Companion Products

Human Endothelial Cell Lysate RUO
500 µg Cat No: 611450

HRP Goat Anti-Mouse Ig RUO
1 mL Cat No: 554002

FITC Goat Anti-Mouse Ig Polyclonal RUO
0.5 mg Cat No: 554001

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


For specific Westbern Blot methodology, please refer to the protocols under "Cell Biology" at our website, http://www.bdbiosciences.com/us/s/resources.


  1. Buitenhuis M, Baltus B, Lammers JW, Coffer PJ, Koenderman L. Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells. Blood. 2003; 101(1):134-142. View reference

  2. Chughtai N, Schimchowitsch S, Lebrun JJ, Ali S. Prolactin induces SHP-2 association with Stat5, nuclear translocation, and binding to the beta-casein gene promoter in mammary cells. J Biol Chem. 2002; 277(34):31107-31114. View reference

  3. Gouilleux F, Wakao H, Mundt M, Groner B. Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription. EMBO J. 1994; 13(18):4361-4369. View reference

  4. Kirken RA, Malabarba MG, Xu J, et al. Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes. J Biol Chem. 1997; 272(22):14098-14103. View reference

  5. Sadowski CL, Choi TS, Le M, Wheeler TT, Wang LH, Sadowski HB. Insulin Induction of SOCS-2 and SOCS-3 mRNA expression in C2C12 Skeletal Muscle Cells Is Mediated by Stat5. J Biol Chem. 2001; 276(23):20703-20710. View reference

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