FITC Mouse Anti-Human CD30
Clone Ber-H83 (RUO (GMP))
- Concentration 3 µg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Co Cells
- Entrez Gene ID 943
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO (GMP)
Regulatory Status Legend
CD30, clone Ber-H83, is derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with Co cells.
CD30 recognizes a 120-kilodalton (kd) type I transmembrane glycoprotein. The CD30 antigen belongs to the tumor necrosis factor receptor/nerve growth factor receptor (TNFR/NGFR) superfamily.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The FITC and PE conjugates are each supplied as 3 μg in 1.0 mL (3 μg/mL) of phosphate-buffered saline (PBS). PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.