Multicolor flow cytometric analysis of adult mouse spleen cells and bone marrow hematopoietic stem cells. (Panel A) BALB/c spleen cells were stained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553090/553089/561878) and Alexa Fluor® 647 Rat Anti-Mouse CD150 (Cat. No. 562647), staining cells well above background compared to Alexa Fluor® 647 Rat IgG2a, κ isotype control (Cat. No. 557690) (data not shown). A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by events with the forward and side-light scattering characteristics of viable lymphocytes. (Panel B) BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE Hamster Anti-Mouse CD48 (Cat. No. 557485/562398), and Alexa Fluor® 647 Rat Anti-Mouse CD150 antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P5 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated). Lineage-negative and CD48- CD150+ (Middle Plot, P4 Gate) cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of adult mouse spleen cells and bone marrow hematopoietic stem cells. (Panel A) BALB/c spleen cells were stained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553090/553089/561878) and Alexa Fluor® 647 Rat Anti-Mouse CD150 (Cat. No. 562647), staining cells well above background compared to Alexa Fluor® 647 Rat IgG2a, κ isotype control (Cat. No. 557690) (data not shown). A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by events with the forward and side-light scattering characteristics of viable lymphocytes. (Panel B) BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE Hamster Anti-Mouse CD48 (Cat. No. 557485/562398), and Alexa Fluor® 647 Rat Anti-Mouse CD150 antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P5 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated). Lineage-negative and CD48- CD150+ (Middle Plot, P4 Gate) cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
製品詳細
BD Pharmingen™
SLAM; Slamf1; Signaling lymphocytic activation molecule family member 1
Mouse (QC Testing)
Rat IgG2a, κ
Mouse CD150 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
27218
AB_2737701
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
557690
V450 Mouse Lineage Antibody Cocktail, with Isotype Control RUO
サイズ
100 Tests
カタログ番号
561301
PE Rat Anti-Mouse CD45R/B220 RUO
サイズ
25 µg
カタログ番号
561878
FITC Rat Anti-Mouse CD41 RUO
サイズ
50 µg
カタログ番号
561849
562647 Rev. 3
抗体の詳細
Q38-480
The Q38-480 monoclonal antibody specifically binds to mouse CD150, also known as SLAM (signaling lymphocyte activation molecule). CD150 is a type 1 transmembrane glycoprotein that is a member of the CD2 subfamily of the Ig superfamily. It is encoded by the Slamf1 (signaling lymphocytic activation molecule family member 1) gene. CD150 is differentially expressed on subsets of thymocytes, T and B lymphocytes, dendritic cells, macrophages, and endothelial cells. SLAM plays multiple roles in innate and adaptive immunity serving as an adhesion molecule and/or coreceptor. CD150-mediated costimulation of TCR-activated T cells reportedly results in the increased production of IFN-γ by Th1 cells and is required for IL-4 production by T follicular helper cells. CD150 also plays important roles in hematopoietic cell developmental pathways. CD150 is differentially expressed by self-renewing adult hematopoietic stem cells (HSC) whereas non-multipotent hematopoietic progenitor cells are CD150-. Utilizing additional cell surface markers, lineage-negative CD150+CD48-CD41- cell fractions are reported to be highly enriched for adult HSC.
562647 Rev. 3
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
562647 Rev.3
引用＆参考文献
Development References (4)
-
Castro AG, Hauser TM, Cocks BG, et al. Molecular and functional characterization of mouse signaling lymphocytic activation molecule (SLAM): differential expression and responsiveness in Th1 and Th2 cells.. J Immunol. 1999; 163(11):5860-70. (Biology). View Reference
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Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005; 121(7):1109-1121. (Biology). View Reference
-
Wang N, Satoskar A, Faubion W, et al. The cell surface receptor SLAM controls T cell and macrophage functions. J Exp Med. 2004; 199(9):1255-1264. (Biology). View Reference
-
Yusuf I, Kageyama R, Monticelli L, et al. Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150). J Immunol. 2010; 185(1):190-202. (Biology). View Reference
562647 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
