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      FITC Mouse Anti-Human P-glycoprotein (CD243)
      FITC Mouse Anti-Human P-glycoprotein (CD243)
      Flow cytometric profile of CD243 expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were stained with either FITC Mouse IgG2b, ĸ Isotype Control (Cat. No. 555742; dashed line histogram) or FITC Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557002; solid line histogram). Fluorescent histograms were derived from gated events with the forward and side-scattering characteristics of viable cells. Flow cytometric analysis was analyzed on a BD FACScan™.
      FITC Mouse Anti-Human P-glycoprotein (CD243)
      Flow cytometric profile of CD243 expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were stained with either FITC Mouse IgG2b, ĸ Isotype Control (Cat. No. 555742; dashed line histogram) or FITC Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557002; solid line histogram). Fluorescent histograms were derived from gated events with the forward and side-scattering characteristics of viable cells. Flow cytometric analysis was analyzed on a BD FACScan™.
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      BD Pharmingen™
      CD243; ABCB1; ABC20; CLCS; GP170; MDR1; P-GP; PGY1
      Human (QC Testing)
      Mouse BALB/c IgG2b, κ
      Human MDR1 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      20 µl
      5243
      AB_396548
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      557002 Rev. 9
      抗体の詳細
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      17F9

      The 17F9 monoclonal antibody specifically binds to the 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody is able to partially block the binding  of UIC2 antibody (another MDR-specific monoclonal antibody).

      557002 Rev. 9
      フォーマットの詳細
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      557002 Rev.9
      引用＆参考文献
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      Development References (3)

      1. Benard J, Bourhis J, Riou G. Clinical significance of multiple drug resistance in human cancers. Anticancer Res. 1990; 10(5A):1297-1302. (Biology). View Reference
      2. Goldstein LJ, Galski H, Fojo A, et al. Expression of a multidrug resistance gene in human cancers. J Natl Cancer Inst. 1989; 81(2):116-124. (Biology). View Reference
      3. Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). View Reference
      557002 Rev. 9

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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