Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      FITC Mouse Anti-Human CD97
      FITC Mouse Anti-Human CD97
      Flow cytometric analysis of CD97 expression on human peripheral blood lymphocytes. PHA-activated (bold line histogram) and resting (light line histogram) lymphocytes were stained with FITC Mouse Anti-Human CD97 (Cat. No. 555773). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.
      FITC Mouse Anti-Human CD97
      Flow cytometric analysis of CD97 expression on human peripheral blood lymphocytes. PHA-activated (bold line histogram) and resting (light line histogram) lymphocytes were stained with FITC Mouse Anti-Human CD97 (Cat. No. 555773). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.
      製品詳細
      Down Arrow Up Arrow


      BD Pharmingen™
      ADGRE5: BL-KDD/F12; TL7LN1
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      20 µl
      V A046, BP030
      AB_396109
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow
      555773 Rev. 7
      抗体の詳細
      Down Arrow Up Arrow
      VIM3b

      The VIM3b monoclonal antibody recognizes CD97. CD97 is a seven-span transmembrane protein that belongs to the G-protein coupled receptor superfamily. CD97 is encoded by ADGRE5 (adhesion G protein-coupled receptor E5). CD97 is highly expressed on monocytes and granulocytes. It is also expressed on dendritic cells, T cells, B cells, NK cells, and smooth muscle cells. CD97 expression is upregulated on activated T cells. CD97 is also expressed on several cell lines including HL-60, THP-1, and K562. CD97 reportedly plays roles in leucocyte signaling, adhesion, and migration. The VIM3b antibody works in Western blotting and immunoprecipitation. The antibody recognizes proteins of 74, 80 and 89 kDa. CD55 and chondroitin sulfate are ligands for CD97.

      555773 Rev. 7
      フォーマットの詳細
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555773 Rev.7
      引用＆参考文献
      Down Arrow Up Arrow

      Development References (1)

      1. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      555773 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback