Purified Mouse Anti-E-Cadherin

BD Transduction Laboratories™ Purified Mouse Anti-E-Cadherin

Name: Purified Mouse Anti-E-Cadherin
Brand: BD Transduction Laboratories™
SKU: 610181

クローン 36/E-Cadherin

(RUO)

RIGHT IMAGE: Immunofluorescent staining of WIDR cells at 1:50 dilution using Purified Mouse Anti-E-Cadherin. LEFT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182). E-Cadherin is observable at 120kDa. Left Panel: A431 lysate (ATCC CRL-1555; Human epithelial carcinoma) was blotted at 1:10000 & 1:20000 (Lanes 1 & 2 respectively; 30 second exposure). Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 (Lanes 1 & 2 respectively; 30 second exposure). Middle Right Panel: 293F cells transfected with human E-Cadherin (CDH1) was blotted at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure). Right Panel: 293 cells transfected with human P-Cadherin (CDH3) was blotted using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182) at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure).

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製品詳細

Brand: BD Transduction Laboratories™

Alternative Name: CD324; CDH1; CADH1; Cadherin-1; ECAD; CDHE; Arc-1; LCAM; UVO; Uvomorulin

Reactivity: Human (QC Testing), Mouse,Rat,Dog (Tested in Development)

Isotype: Mouse IgG2a, κ

Immunogen: Human E-Cadherin C-terminal Recombinant Protein

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)

Target Molecular Weight: 120 kDa

Concentration: 250 µg/ml

RRID: AB_397581

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610181 Rev. 3

抗体の詳細

36/E-Cadherin

E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. The 36/E-Cadherin monoclonal antibody recognizes the cytoplasmic domain of E-Cadherin, regardless of phosphorylation status. The peptide immunogen was generated from human E-Cadherin aa. 735-883.

Note: Investigators are advised that this antibody has some degree of cross-reactivity to P-Cadherin.

610181 Rev. 3

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

610181 Rev.3

引用＆参考文献

Development References (5)

Jaksits S, Kriehuber E, Charbonnier AS, Rappersberger K, Stingl G, Maurer D. CD34+ cell-derived CD14+ precursor cells develop into Langerhans cells in a TGF-beta 1-dependent manner. J Immunol. 1999; 163(9):4869-4877. (Clone-specific: Flow cytometry). View Reference
Miyoshi K, Shillingford JM, Smith GH, et al. Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium. J Cell Biol. 2001; 155(4):531-542. (Clone-specific: Immunohistochemistry). View Reference
Sheibani N, Sorenson CM, Frazier WA. Differential modulation of cadherin-mediated cell-cell adhesion by platelet endothelial cell adhesion molecule-1 isoforms through activation of extracellular regulated kinases. Mol Biol Cell. 2000; 11(8):2793-2802. (Clone-specific: Immunofluorescence, Western blot). View Reference
Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.. Development. 1988; 102(4):639-55. (Biology). View Reference
Weng Z, Xin M, Pablo L, et al. Protection against anoikis and down-regulation of cadherin expression by a regulatable beta-catenin protein. J Biol Chem. 2002; 277(21):18677-18686. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference

すべて表示する (5)

610181 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.