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Purified Mouse Anti-Human RANTES
Purified Mouse Anti-Human RANTES
Immunocytochemical staining for RANTES.  PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma Cat. No. L-8274, 1 µg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained  by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 550337) and the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) (Nomarski optics, original magnification 400X).
Immunocytochemical staining for RANTES.  PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37°C with LPS (Sigma Cat. No. L-8274, 1 µg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained  by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody (Cat. No. 550337) and the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) (Nomarski optics, original magnification 400X).
製品詳細
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BD Pharmingen™
CCL5; chemokine (C-C motif) ligand 5; EoCP; SCYA5; SIS-delta; SISd; TCP228
Human (QC Testing)
Mouse BALB/c IgG1, κ
Recombinant Human RANTES Protein
Intracellular staining (flow cytometry) (Routinely Tested), Immunocytochemistry (Tested During Development)
0.5 mg/ml
AB_2290967
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550420 Rev. 3
抗体の詳細
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2D5

The 2D5 monoclonal antibody specifically binds to human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). RANTES belongs to the C-C family of chemokines and is also known as CCL5. RANTES is produced by activated macrophages, CD8+ T cells, platelets, fibroblasts, epithelial cells and some tumor cells. RANTES is a chemoattractant and response modifier for a variety of cells including memory T cells, NK cells, dendritic cells, monocytes, eosinophils, basophils, and mast cells. RANTES binds to and transduces intracellular signals through several cell surface chemokine receptors including CCR1, CCR3, CCR4, and CCR5. RANTES can reportedly inhibit the interaction between certain HIV viruses and CCR5 and thus suppress viral infection in vitro.

550420 Rev. 3
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550420 Rev.3
引用&参考文献
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Development References (4)

  1. Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol. 1981; 75(5):734-738. (Methodology). View Reference
  2. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-580. (Methodology). View Reference
  3. Schall TJ, Jongstra J, Dyer BJ, et al. A human T cell-specific molecule is a member of a new gene family. J Immunol. 1988; 141(3):1018-1025. (Biology). View Reference
  4. Sticherling M, Küpper M, Koltrowitz F, et al. Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts.. J Invest Dermatol. 1995; 105(4):585-91. (Biology). View Reference
すべて表示する (4) 表示項目を減らす
550420 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.