Two-color flow cytometric analysis of Oct-2 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMCs) and BALB/c mouse splenic leucocytes were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (562574/562725). Cells were then stained with Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557906) or Alexa Fluor® 647 Rat Anti-Oct-2 antibody (Cat. No. 563472). Human PBMCs (Left Panels) were counterstained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741). Mouse splenocytes (Right Panels) were counterstained with Alexa Fluor® 488 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557669). The two-color flow cytometric dot plots show the correlated expression of Oct-2 (or Ig Isotype control staining) versus human CD19 (Left Panels) or mouse CD45R/B220 (Right Panels) for gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of Oct-2 expression in human and mouse leucocytes. Human peripheral blood mononuclear cells (PBMCs) and BALB/c mouse splenic leucocytes were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (562574/562725). Cells were then stained with Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557906) or Alexa Fluor® 647 Rat Anti-Oct-2 antibody (Cat. No. 563472). Human PBMCs (Left Panels) were counterstained with PE Mouse Anti-Human CD19 antibody (Cat. No. 555413/561741). Mouse splenocytes (Right Panels) were counterstained with Alexa Fluor® 488 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557669). The two-color flow cytometric dot plots show the correlated expression of Oct-2 (or Ig Isotype control staining) versus human CD19 (Left Panels) or mouse CD45R/B220 (Right Panels) for gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
製品詳細
BD Pharmingen™
Oct2, OTF-2, OCT2, OTF2, Oct2, NF-A2, POU2F2, Pou2f2, Otf2
Mouse (QC Testing), Human (Tested in Development)
Rat IgG2a, κ
Mouse Oct-2 amino acids 1-44 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2738229
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Transcription Factor Buffer Set RUO
サイズ
100 Tests
カタログ番号
562574
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
サイズ
100 Tests
カタログ番号
557906
Transcription Factor Buffer Set RUO
サイズ
25 Tests
カタログ番号
562725
PE Mouse Anti-Human CD19 RUO
サイズ
100 Tests
カタログ番号
555413
PE Mouse Anti-Human CD19 RUO
サイズ
25 Tests
カタログ番号
561741
563472 Rev. 1
抗体の詳細
9A2
The 9A2 monoclonal antibody specifically binds to Oct-2 that has a molecular weight of approximately 50-60 kDa. The 9A2 antibody was generated against the 44 N-terminal amino acids common to mouse Oct-2 isoforms and crossreacts with both human and mouse Oct-2. Oct-2 is a POU class 2 homeobox 2 transcription factors (POU2F2) that is also known as OCT2, OTF2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 and Octamer-binding transcription factor 2. The POU domain in the Oct-2 protein is required for low and high affinity cooperative binding of the octomer sequence and heptamer site in immunoglobulin promoters and promoters for other genes. Oct-2 is expressed in B cells, activated CD4+ and CD8+ T cells, monocytes/macrophages and subsets of dendritic cells. Oct-2 is reportedly associated with neoplasms including Diffuse Large B-cell lymphoma and Nodular Lymphocyte predominant Hodgkin's lymphoma. Studies with knockout mouse models suggest Oct-2 is required for B cell maturation, but not for B cell commitment, and for B cell production of IL-6.
563472 Rev. 1
フォーマットの詳細
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
563472 Rev.1
引用＆参考文献
Development References (8)
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Borestrom C, Forsman A, Ruetschi U, Rymo L. E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. J Gen Virol. 2012; 93(Ot 5):1065-1075. (Biology). View Reference
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Browne P, Petrosyan K, Hernandez A, Chan JA. The B-cell transcription factors BSAP, Oct-2, and BOB.1 and the pan-B-cell markers CD20, CD22, and CD79a are useful in the differential diagnosis of classic Hodgkin lymphoma. Am J Clin Pathol. 2003; 120(5):767-777. (Biology). View Reference
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Chou YY, Gao JI, Chang SF, Chang PY, Lu SC. Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2. FEBS J. 2011; 278(1):85-96. (Biology). View Reference
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Corcoran LM, Koentgen F, Dietrich W, Veale M, Humbert PO. All known in vivo functions of the Oct-2 transcription factor require the C-terminal protein domain. J Immunol. 2004; 172(5):2962-2969. (Clone-specific: Gel shift, Western blot). View Reference
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Herbeck R, Teodorescu Brinzeu D, Giubelan M, Lazar E, Dema A, Ionita H. B-cell transcription factors Pax-5, Oct-2, BOB.1, Bcl-6, and MUM1 are useful markers for the diagnosis of nodular lymphocyte predominant Hodgkin lymphoma. Rom J Morphol Embryol. 2011; 52(1):69-74. (Biology). View Reference
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Kang SM, Tsang W, Doll S, et al. Induction of the POU domain transcription factor Oct-2 during T-cell activation by cognate antigen. Mol Cell Biol. 1992; 12(7):3149-3154. (Biology). View Reference
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Karnowski A, Chevrier S, Belz GT, et al. B and T cells collaborate in antiviral responses via IL-6, IL-21, and transcriptional activator and coactivator, Oct2 and OBF-1. J Exp Med. 2012. (Clone-specific: Gel shift, Western blot). View Reference
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Saglam A, Uner AH. Immunohistochemical expression of Mum-1, Oct-2 and Bcl-6 in systemic anaplastic large cell lymphomas. Tumori. 2011; 97(5):634-638. (Biology). View Reference
563472 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
