FITC Mouse Anti-Human CD10
Clone W8E7 (体外診断用医薬品)
- Alternative Name CALLA
- Concentration 6 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Blasts from a donor with non-T ALL
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status 体外診断用医薬品
Regulatory Status Legend
The CD10 antibody, clone W8E7, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with non-T ALL.
The CD10 antibody (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen (CALLA), with a molecular weight of 100 kilodaltons (kDa). The CD10 antigen is identical to human membrane–associated neutral endopeptidase (NEP; EC 18.104.22.168), also known as enkephalinase.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline— Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.