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PE Rat Anti-Mouse CD43
PE Rat Anti-Mouse CD43
Flow cytometric analysis of CD43 expression on mouse splenocytes. BALB/c splenocytes were stained with either PE Rat Anti-Mouse CD43 (Cat. No. 553271/561857; solid line histogram) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). Fluorescence histograms depicting CD43 (or Ig isotype control) expression were derived from side and forward light-scatter characteristics of viable splenocytes.  
Flow cytometric analysis of CD43 expression on mouse splenocytes. BALB/c splenocytes were stained with either PE Rat Anti-Mouse CD43 (Cat. No. 553271/561857; solid line histogram) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). Fluorescence histograms depicting CD43 (or Ig isotype control) expression were derived from side and forward light-scatter characteristics of viable splenocytes.  
製品詳細
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BD Pharmingen™
Spn; Sialophorin; Leukosialin; Ly-48; Ly48; Galgp; LEUK
Mouse (QC Testing)
Rat DA x LOU IgG2a, κ
Mouse Plasmacytoma MOPC-315
Flow cytometry (Routinely Tested)
0.2 mg/ml
20737
AB_10926206
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
561857 Rev. 2
抗体の詳細
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S7

The S7 monoclonal antibody specifically binds to the 115 kDa glycosylated form of CD43 (Ly-48, Leukosialin). CD43 is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion.

561857 Rev. 2
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561857 Rev.2
引用&参考文献
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Development References (12)

  1. Gulley ML, Ogata LC, Thorson JA, Dailey MO, Kemp JD. Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation. J Immunol. 1988; 140(11):3751-3757. (Immunogen). View Reference
  2. Hardy R, Hayakawa K. Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting. Ann N Y Acad Sci. 1992; 651:99-111. (Biology). View Reference
  3. Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med. 1991; 173(5):1213-1225. (Biology). View Reference
  4. Jones AT, Federsppiel B, Ellies LG, et al. Characterization of the activation-associated isoform of CD43 on murine T lymphocytes. J Immunol. 1994; 153(8):3426-3439. (Biology). View Reference
  5. Manjunath N, Correa M, Ardman M, Ardman B. Negative regulation of T-cell adhesion and activation by CD43. Nature. 1995; 377(6549):535-538. (Biology). View Reference
  6. Moore T, Bennett M, Kumar V. Transplantable NK cell progenitors in murine bone marrow. J Immunol. 1995; 154(4):1653-1663. (Biology). View Reference
  7. Moore T, Huang S, Terstappen LW, Bennett M, Kumar V. Expression of CD43 on murine and human pluripotent hematopoietic stem cells. J Immunol. 1994; 153(11):4978-4987. (Biology). View Reference
  8. Rolink A, ten Boekel E, Melchers F, Fearon DT, Krop I, Andersson J. A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors. J Exp Med. 1996; 183(1):187-194. (Biology). View Reference
  9. Stockton BM, Cheng G, Manjunath N, Ardman B, von Andrian UH. Negative regulation of T cell homing by CD43. Immunity. 1998; 8(3):373-381. (Biology). View Reference
  10. Thurman EC, Walker J, Jayaraman S, Manjunath N, Ardman B, Green JM. Regulation of in vitro and in vivo T cell activation by CD43. Int Immunol. 1998; 10(5):691-701. (Biology). View Reference
  11. Wells SM, Kantor AB, Stall AM. CD43 (S7) expression identifies peripheral B cell subsets. J Immunol. 1994; 153(12):5503-5515. (Biology). View Reference
  12. Woodman RC, Johnston B, Hickey MJ, et al. The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient mice. J Exp Med. 1998; 188(11):2181-2186. (Biology). View Reference
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561857 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.