FITC Mouse Anti-Mouse H-2D[k]

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BD Pharmingen™ FITC Mouse Anti-Mouse H-2D[k]

Name: FITC Mouse Anti-Mouse H-2D[k]
Brand: BD Pharmingen™
SKU: 553585

クローン 15-5-5

(RUO)

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製品詳細

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Mouse C3H.SW IgG2a, κ

Immunogen: C3H mouse splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_394939

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

We have observed that the staining intensity of FITC-conjugated 15-5-5 mAb is severely reduced after fixation of stained leukocytes (≤ 1 hour with 1% formaldehyde). Therefore, one should not fix the stained cells prior to flow cytometry. We have found that freshly-isolated leukocytes and cell lines may wait for analysis in wash buffer at 4°C, without fixation, for up to 18 hours post-staining without loss of viability. Activated lymphocytes may lose viability rapidly, and data should be collected within 5 hours post-staining.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

FITC Mouse IgG2a, κ Isotype Control RUO

サイズ 0.25 mg カタログ番号 553456

553585 Rev. 9

抗体の詳細

15-5-5

The 15-5-5 monoclonal antibody specifically recognizes the H-2D[k] MHC class I alloantigen. It weakly cross-reacts with H-2K[d] and with cells from mice of the H-2[f] haplotype. Reactivity with other haplotypes (e.g., a, b, p, q, r, s) has not been observed.

553585 Rev. 9

フォーマットの詳細

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: FITC

励起源: Blue 488 nm

最大励起波長: 494 nm

最大蛍光波長: 518 nm

553585 Rev.9

引用＆参考文献

Development References (1)

Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980; 124(2):533-540. (Immunogen). View Reference

553585 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.