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Purified Mouse Anti-Human CD79b 3A2-2E7 (also known as SN8) RUO

Purified Mouse Anti-Human CD79b 

Clone  3A2-2E7 (also known as SN8)   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Igβ; B29; IGB; AGM6; CD79B
  • Concentration 0.5 mg/ml
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Cell membranes from human B-prol ymphocytic leukemia (B-PLL) cells
  • Workshop No. V B037
  • Entrez Gene ID 974 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The 3A2-2E7 monoclonal antibody (also known as SN8) specifically recognizes CD79b. Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Igá and Igâ, clustered as CD79a and CD79b, respectively. CD79b is a membrane glycoprotein of 229 residues, with a predicted relative molecular mass of 36-40 kDa. Its expression is restricted to B lineage cells. CD79b reportedly associates with surface IgM and is involved in signal transduction. The 3A2-2E7 antibody has similar reactivity characteristics as clone CB3-1. The 3A2-2E7 and CD3-1 antibodies specifically react with an epitope that is enhanced on certain B-cell leukemias such as prolymphocytic leukemia and lymphoma, but not on chronic lymphocytic leukemia.

Format
  • Format Purified

Other Formats →

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500 mL Cat No: 554656

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PE Mouse Anti-Human CD19 HIB19 RUO
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Lysing Buffer RUO
100 mL Cat No: 555899

Lysing Solution 10X Concentrate RUO (GMP)
100 Cat No: 349202

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.


  1. Astsaturov IA, Matutes E, Morilla R, et al. Differential expression of B29 (CD79b) and mb-1 (CD79a) proteins in acute lymphoblastic leukaemia. Leukemia. 1996; 10(5):769-773. View reference

  2. Cragg MS, Chan HT, Fox MD, et al. The alternative transcript of CD79b is overexpressed in B-CLL and inhibits signaling for apoptosis. Blood. 2002; 100(9):3068-3076. View reference

  3. Moreau EJ, Matutes E, A'Hern RP, et al. Improvement of the chronic lymphocytic leukemia scoring system with the monoclonal antibody SN8 (CD79b). Am J Clin Pathol. 1997; 108(4):378-382. View reference

  4. Okazaki M, Luo Y, Han T, Yoshida M, Seon BK. Three new monoclonal antibodies that define a unique antigen associated with prolymphocytic leukemia/non-Hodgkin's lymphoma and are effectively internalized after binding to the cell surface antigen. Blood. 1993; 81(1):84-94. View reference

  5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

  6. Van Kooten C, Galibert L, Seon BK, Garrone P, Liu YJ, Banchereau J. Cross-linking of antigen receptor via Ig-beta (B29, CD79b) can induce both positive and negative signals in CD40-activated human B cells. Clin Exp Immunol. 1997; 110(3):509-515. View reference

  7. Zomas AP, Matutes E, Morilla R, Owusu-Ankomah K, Seon BK, Catovsky D. Expression of the immunoglobulin-associated protein B29 in B cell disorders with the monoclonal antibody SN8 (CD79b). Leukemia. 1996; 10(12):1966-1970. View reference

557592 Rev. 4
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