FITC Mouse Anti-Human CD79b
Clone 3A2-2E7 (also known as SN8) (RUO)
- Concentration 25 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Cell membranes from human B-prol ymphocytic leukemia (B-PLL) cells
- Workshop No. V B037
- Entrez Gene ID 974
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The CD79b antibody, clone SN8 (3A2-2E7-1F5), is generated from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with cell membrane preparations from B-prolymphocytic leukemia (B-PLL) cells.
The CD79b antibody recognizes an epitope on the extracellular domain of a 36–40 kilodalton (kDa) type I membrane glycoprotein. Immunoglobulin (Ig) antigen receptors are composed of a non-covalently associated complex of Ig and two other proteins, Igα and Igβ, which have been designated as CD79a and CD79b, respectively.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.