Purified Mouse Anti- NFAT-1
Clone 1/NFAT-1 (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name NF-ATc2; NFATP; KIAA0611
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing) Dog (Tested in Development)
Western blot (Routinely Tested)
Immunofluorescence, Immunoprecipitation (Tested During Development)
- Immunogen Human NFAT-1 aa. 29-181
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.
Suggested Companion Products
Preparation and Storage
Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml