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Purified Mouse Anti- NFAT-1 1/NFAT-1 RUO

Purified Mouse Anti- NFAT-1 

Clone  1/NFAT-1   (RUO)

  • Brand BD Transduction Laboratories™
  • Alternative Name NF-ATc2; NFATP; KIAA0611
  • Concentration 250 µg/ml
  • Isotype Mouse IgG1
  • Reactivity Human (QC Testing)  Dog (Tested in Development) 
  • Application Western blot (Routinely Tested) 
    Immunofluorescence, Immunoprecipitation (Tested During Development) 
  • Immunogen Human NFAT-1 aa. 29-181
  • Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

FITC Mouse Anti- NFAT-1 1/NFAT-1 RUO
50 µg Cat No: 611960

Jurkat Cell Lysate RUO
500 µg Cat No: 611451

MDCK Cell Lysate RUO
500 µg Cat No: 611635

HRP Goat Anti-Mouse Ig RUO
1 mL Cat No: 554002

FITC Goat Anti-Mouse Ig Polyclonal RUO
0.5 mg Cat No: 554001

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml


  1. Boise LH, Petryniak B, Mao X, et al. The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB. Mol Cell Biol. 1993; 13(3):1911-1919. View reference

  2. Good L, Maggirwar SB, Harhaj EW, Sun SC. Constitutive dephosphorylation and activation of a member of the nuclear factor of activated T cells, NF-AT1, in Tax-expressing and type I human T-cell leukemia virus-infected human T cells. J Biol Chem. 1997; 272(3):1425-1428. View reference

  3. Grader-Beck T, van Puijenbroek AA, Nadler LM, Boussiotis VA. cAMP inhibits both Ras and Rap1 activation in primary human T lymphocytes, but only Ras inhibition correlates with blockade of cell cycle progression. Blood. 2003; 101(3):998-1006. View reference

  4. Kadereit S, Mohammad SF, Miller RE, et al. Reduced NFAT1 protein expression in human umbilical cord blood T lymphocytes. Blood. 1999; 94(9):3101-3107. View reference

  5. McCaffrey PG, Luo C, Kerppola TK, et al. Isolation of the cyclosporin-sensitive T cell transcription factor NFATp. Science. 1993; 262(5134):750-754. View reference

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