FITC Mouse Anti- NFAT-1
Clone 1/NFAT-1 (RUO)
- Product Announcement
- Brand BD Transduction Laboratories™
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Dog (QC Testing) Human (Tested in Development)
Immunofluorescence (Routinely Tested)
- Immunogen Human NFAT-1 aa. 29-181
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.
This antibody is routinely tested by immunoflouresence microscopy. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.