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Biotin Rat Anti-Mouse IgG3 R40-82 RUO

Biotin Rat Anti-Mouse IgG3 

Clone  R40-82   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Ighg3; Immunoglobulin heavy constant gamma 3
  • Concentration 0.5 mg/ml
  • Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
  • Reactivity Mouse (QC Testing) 
  • Application ELISA (Routinely Tested) 
    Flow cytometry (Tested During Development) 
    Immunohistochemistry-frozen (Reported) 
  • Immunogen Mouse Pooled Ig
  • Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.

Format
  • Format Biotin

Other Formats →

Suggested Companion Products

FITC Rat Anti-Mouse IgG3 R40-82 RUO
0.5 mg Cat No: 553403

Purified Rat Anti-Mouse IgG3 R2-38 RUO
0.5 mg Cat No: 553404

Purified Mouse IgG3, κ Isotype Control RUO
0.5 mg Cat No: 553486

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


For the sandwich mouse IgG3, ELISA, Biotin Rat Anti-Mouse IgG3 is optimal for detection with Purified Rat Anti-Mouse IgG3 (Cat. No. 553404) for capture and Purified Mouse IgG3, κ Isotype Control (Cat. No. 553486) for the standard. Biotin Rat Anti-Mouse IgG3 may also be used as a primary or secondary reagent in immunofluorescent staining. Biotin Rat Anti-Mouse IgG3 is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG3, we recommend FITC Rat Anti-Mouse IgG3 (Cat. No. 553403).


  1. Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. View reference

  2. Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. View reference

553401 Rev. 15
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