Clone UCHL1 (RUO)
- Concentration 50 μg/mL
- Vol. Per Test 20 μL
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human IL-2-dependent T-cell line
- Workshop No. IV N31
- Entrez Gene ID 5788
- Regulatory Status RUO
Regulatory Status Legend
The CD45RO antibody, clone UCHL-1, is derived from hybridization of mouse P3/NS-1/1-Ag4-1 myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)–dependent human T-cell line.
The CD45RO antibody recognizes a 180-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). CD45 is a member of the CD45 family that includes CD45, CD45RA, and CD45RB. The CD45 antigen is a protein tyrosine phosphatase.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.