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      Purified Rat Anti-Mouse Caspase-11
      Purified Rat Anti-Mouse Caspase-11
      Western blot analysis of Caspase-11 protein expression by mouse J774 cells. Lysate was prepared from cells of the mouse J774 macrophage cell line. Cell lysate proteins were resolved under reducing conditions by electrophoresis (SDS-PAGE) in a 4-20% Tris-Glycine polyacrylamide gel and transferred to a PVDF membrane. The blot was then probed with either 0.250 µg/ml (Lane 1), 0.125 µg/ml (Lane 2), or 0.063 µg/ml (Lane 3) of Purified Rat Anti-Mouse Caspase-11 antibody (Cat. No. 564971) followed by detection with HRP-conjugated Goat Anti-Rat Ig (Cat. No. 554017), and ECL Western blot detection reagents. Caspase-11 was identified as protein bands of approximately 38 to 43 kDa.
      Purified Rat Anti-Mouse Caspase-11
      Western blot analysis of Caspase-11 protein expression by mouse J774 cells. Lysate was prepared from cells of the mouse J774 macrophage cell line. Cell lysate proteins were resolved under reducing conditions by electrophoresis (SDS-PAGE) in a 4-20% Tris-Glycine polyacrylamide gel and transferred to a PVDF membrane. The blot was then probed with either 0.250 µg/ml (Lane 1), 0.125 µg/ml (Lane 2), or 0.063 µg/ml (Lane 3) of Purified Rat Anti-Mouse Caspase-11 antibody (Cat. No. 564971) followed by detection with HRP-conjugated Goat Anti-Rat Ig (Cat. No. 554017), and ECL Western blot detection reagents. Caspase-11 was identified as protein bands of approximately 38 to 43 kDa.
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      BD Pharmingen™
      CASP-11; Casp11; CASP-4; Casp4; Caspase-4; Caspl; ich-3; Ich3
      Mouse (QC Testing)
      Rat IgG2a, κ
      Mouse Caspase-11 Recombinant Protein
      Western blot (Routinely Tested)
      38–43 kDa
      0.5 mg/ml
      AB_2739029
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      564971 Rev. 2
      抗体の詳細
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      17D9

      The 17D9 monoclonal antibody specifically binds to mouse Caspase-11 (CASP-11/Casp11) which is also known as ICH-3. Mouse Caspase-11 is homologous to human Caspase-4 and is encoded by the mouse Casp4 (Caspase 4, apoptosis-related cysteine peptidase) gene. Caspase-11 is a cysteine protease that functions in apoptosis and inflammatory responses. Caspase-11 exists as 38 and 43 kDa pro-caspase forms, and as 30 and 10 kDa active forms. Caspase-11 is an initiator caspase that activates other caspases, including Caspase-1 [IL-1β-converting enzyme (ICE)], Caspase-3, and Caspase-7, which are involved in executing apoptosis. When Caspase-11 is deficient, cells are unable to process or secrete Interleukin-1β (IL-1β) and demonstrate defective motility in response to certain stimuli. Unlike other caspases that are expressed constitutively, Caspase-11 expression can be upregulated in cells stimulated with certain activators, e.g., macrophages, lymphocytes and hepatocytes stimulated with lipopolysaccharide.

      564971 Rev. 2
      フォーマットの詳細
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      564971 Rev.2
      引用＆参考文献
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      Development References (8)

      1. Kang SJ, Wang S, Hara H, et al. Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions. J Cell Biol. 2000; 149(3):613-622. (Clone-specific: Western blot). View Reference
      2. Kayagaki N, Warming S, Lamkanfi M et al. Non-canonical inflammasome activation targets caspase-11. Nature. 2011; 479(7371):117-121. (Clone-specific: Western blot). View Reference
      3. Li J, Brieher WM, Scimone ML, et al. Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. Nat Cell Biol. 2007; 9(3):276-286. (Clone-specific: Western blot). View Reference
      4. Li J, Yin HL, Yuan J. Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity. J Cell Biol. 2008; 181(2):321-333. (Clone-specific: Western blot). View Reference
      5. Meunier E, Dick MS, Dreier RF, et al. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPase. Nature. 2014; 509(7500):366-370. (Clone-specific: Western blot). View Reference
      6. Shi J, Zhao Y, Wang Y, et al. Inflammatory caspases are innate immune receptors for intracellular LPS. Nature. 2014; 514:187-192. (Clone-specific). View Reference
      7. Wang S, Miura M, Jung Y, et al. Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE. J Cell Biol. 1996; 271(34):20580-20587. (Immunogen: Western blot). View Reference
      8. Wang S, Miura M, Jung YK, Zhu H, Li E, Yuan J. Murine caspase-11, an ICE-interacting protease, is essential for the activation of ICE. Cell. 1998; 92(4):501-509. (Clone-specific: Western blot). View Reference
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      564971 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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