Preparation and Storage
推奨アッセイ手順
Applications include western blot analysis (1:1000-1:4000). Mouse lung lysate is recommended as a positive control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Apoptosis is essential in maintaining normal cellular homeostasis and for animal development. Morphological as well as biochemical changes are a hallmark of apoptosis and include membrane blebbing, chromosomal condensation and DNA fragmentation. These changes are caused by a class of cysteine proteases called caspases, which, upon activation can cleave multiple cytoplasmic and nuclear substrates. Caspases, once activated by a proapoptotic signal can induce a DNase, designated DFF/CAD to cleave these nuclear substrates. In growing, non-apoptotic cells DFF/CAD is complexed with its inhibitor, ICAD. Activation of caspase-3 causes the cleavage of ICAD, releasing CAD to cleave chromosomal DNA. ICAD has a molecular weight of approximately ~45 kDa in SDS-PAGE. The antibody recognizes the full length and cleaved form (~18 kDa) of mouse ICAD. A peptide corresponding to amino acids 312 to 331 of mouse ICAD was used as immunogen.
Development References (4)
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Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature. 1998; 391(6662):43-50. (Immunogen). View Reference
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Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell. 1997; 89(2):175-184. (Biology). View Reference
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Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature. 1998; 391(6662):96-99. (Biology). View Reference
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Sakahira H, Enari M, Nagata S. Functional differences of two forms of the inhibitor of caspase-activated DNase, ICAD-L, and ICAD-S. J Biol Chem. 1999; 274(22):15740-15744. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
