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Purified Mouse Anti-Human Wnt16
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Purified Mouse Anti-Human Wnt16

クローン F4-1582

(RUO)
Purified Mouse Anti-Human Wnt16
Western blot analysis of Wnt16. Lysate from 697 cells was probed with anti-Wnt 16 (clone F4-1582, Cat. No. 552595) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.063 µg/ml (lane 3). Wnt16 is identified as a band of ~40 kDa.
Western blot analysis of Wnt16. Lysate from 697 cells was probed with anti-Wnt 16 (clone F4-1582, Cat. No. 552595) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.063 µg/ml (lane 3). Wnt16 is identified as a band of ~40 kDa.
製品詳細
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BD Pharmingen™
0.5 mg/ml
Human (QC Testing)
Western blot (Routinely Tested)
RUO
AB_394436


説明

The Wnt gene family comprises a large group of signaling proteins involved in developmental pathways in vertebrates, Drosophilia, C. elegans and other organisms. The Wnt genes were first discovered in mouse (called int-1) and later in Drosophilia (called wingless, or wg) where the signaling pathways were unravelled. The Wnt proteins are involved in various developmental processes including embryonic induction, generation of cell polarity and the specification of cell fate. The vertebrate Wnt glycoproteins number at least 16 members and initiate signaling by being secreted and a subset of these glycoproteins bind to a class of receptors, called Frizzled, of which 11 have been identified. Stimulation of the Wnt pathway causes the phosphorylation of Dishevelled, which inhibits glycogen synthase kinase -3β (GSK3β) and allows β-catenin to accumulate in the cytosol. β-catenin then translocates to the nucleus to form a complex with Tcf/LEF family of transcription factors to activate transcription. In unstimulated cells, β-catenin forms a complex with the proteins Axin, adenomatous polyposis coli (APC), (GSK3ß) and is unstable. Studies have shown that some members of this pathway become mutated in human cancers, such as colon carcinoma and melanoma. Futhermore, studies on the WNT-16 gene, have shown that it is activated by the E2A-Pbx fusion product in acute lymphoblastoid leukemia.  Wnt16 has a predicted molecular weight of 40 kDa (SWISS-PROT:Q9UBV4).

調製と保管

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Store the undiluted antibody at 4°C. Store 697 cell lysate at -20°C.

推奨アッセイ手順

Applications include western blot analysis (0.063 - 0.25 µg/ml). 697 cell lysate [50 µg (1 µg/µl)] is provided as a positive control (51-9000020).

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552595 Rev. 4
構成品
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説明 数量/容量 Part Number EntrezGene ID
Purified mouse anti-human WNT16 monoclonal antibody 50 µg (1 ea) 51-9000015 N/A
697 control lysate 50 µg (1 ea) 51-9000020 N/A
552595 Rev. 4
引用&参考文献
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開発者向け参考資料 (5)

  1. Cadigan KM, Nusse R. Wnt signaling: a common theme in animal development. Genes Dev. 1997; 11(24):3286-3305. (Biology). 参考文献を見る
  2. McWhirter JR, Neuteboom ST, Wancewicz EV, Monia BP, Downing JR, Murre C. Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia. Proc Natl Acad Sci U S A. 1999; 96(20):11464-11469. (Biology). 参考文献を見る
  3. Polakis P. Wnt signaling and cancer.. Genes Dev. 2000; 14(15):1837-51. (Biology). 参考文献を見る
  4. Taipale J, Beachy PA. The Hedgehog and Wnt signalling pathways in cancer. Nature. 2001; 411(6835):349-354. (Biology). 参考文献を見る
  5. Wodarz A, Nusse R. Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol. 1998; 14:59-88. (Biology). 参考文献を見る
552595 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.