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Purified Mouse Anti-GAP-43/Neuromodulin
製品詳細
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Human GAP-43/Neuromodulin aa. 1-144
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
43 kDa
250 µg/ml
AB_399580
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612262 Rev. 1
抗体の詳細
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31/GAP-43/Neuromodulin

Neuromodulin (GAP-43, B-50, F-1) is a neural-specific protein that represents about one percent of the total membrane protein in growth cones from developing rat brain. Growth cones are located at the distal tip of neuronal processes and are critical for transduction of extracellular signals into directed growth. Neuromodulin is attached to the membranes of the neuronal growth cones during development. In the adult, it is attached to the presynaptic membranes in discrete subsets of synapses. Neuromodulin can bind to calmodulin, as well as inhibit phosphatidylinositol phosphate kinase (PIP kinase) activity. It is also a prominent substrate of Protein Kinase C (PKC) and may mediate some of the effects of PKC on growth cone function. As may be expected, expression and phosphorylation of neuromodulin are closely associated with axonal outgrowth and repair. Phosphorylation of neuromodulin by PKC has been correlated with the persistence of long term potentiation and the translocation of PKC to synaptosomal membranes. This PKC-mediated phosphorylation does not appear to require neuromodulin membrane association. It has also been demonstrated that the GTP-binding protein, G[o], is a main component of growth cone membranes. The binding of GTP--S to G[o] is stimulated by neuromodulin and this activity resides within the amino-terminal region of the protein. This region of neuromodulin shares sequence homology with the cytoplasmic tails of G-linked transmembrane receptors, suggesting the presence of one binding site on G[o] for both neuromodulin and transmembrane receptors.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612262 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612262 Rev.1
引用&参考文献
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Development References (4)

  1. Chapman ER, Estep RP, Storm DR. Palmitylation of neuromodulin (GAP-43) is not required for phosphorylation by protein kinase C. J Biol Chem. 1992; 267(35):25233-25238. (Biology). View Reference
  2. Cimler BM, Andreasen TJ, Andreasen KI, Storm DR. P-57 is a neural specific calmodulin-binding protein. J Biol Chem. 1985; 260(19):10784-10788. (Biology). View Reference
  3. Meiri KF, Bickerstaff LE, Schwob JE. Monoclonal antibodies show that kinase C phosphorylation of GAP-43 during axonogenesis is both spatially and temporally restricted in vivo. J Cell Biol. 1991; 112(5):991-1005. (Biology). View Reference
  4. Strittmatter SM, Valenzuela D, Kennedy TE, Neer EJ, Fishman MC. G0 is a major growth cone protein subject to regulation by GAP-43. Nature. 1990; 344(6269):836-841. (Biology). View Reference
すべて表示する (4) 表示項目を減らす
612262 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.