Preparation and Storage
推奨アッセイ手順
Western blot: Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
R-Cadherin was first isolated from chicken retina, but was subsequently shown to be present and highly conserved in a variety of tissues from several different species. A 913 amino acid polypeptide, R-Cadherin is highly homologous to Cadherin-4, but is most closely related to another family member, N-Cadherin. In experiments using chimeric L cells expressing chicken or mouse Cadherins, those cells expressing mouse or chicken R-Cadherin were able to aggregate with each other as well as with cells expressing N-Cadherin. However, there was no aggregation of R-Cadherin expressing cells, with P- or E-Cadherin expressing cells. This suggests that the roles of specific cadherins are conserved among types in the determination of heterogenous cell segregation and distribution during tissue morphogenesis and maintenance.
Development References (3)
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Hutton JC, Christofori G, Chi WY, et al. Molecular cloning of mouse pancreatic islet R-cadherin: differential expression in endocrine and exocrine tissue. Mol Endocrinol. 1993; 7(9):1151-1160. (Biology). View Reference
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Matsunami H, Miyatani S, Inoue T, et al. Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci. 1993; 106(1):401-409. (Biology). View Reference
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Tanihara H, Sano K, Heimark RL, St John T, Suzuki S. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhes Commun. 1994; 2(1):15-26. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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