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Purified Mouse Anti-Ubc9
Purified Mouse Anti-Ubc9

Western blot analysis of Ubc9 on human endothelial lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Ubc9.

Western blot analysis of Ubc9 on human endothelial lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Ubc9.

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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human Ubc9 aa. 26-156
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
18 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610748 Rev. 1
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Progression of the mammalian cell cycle is primarily regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an e-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome (a cytoplasmic complex of proteases). Human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been reported, including Ubc9. Ubc9 is 158 amino acids with an apparent molecular weight of 18kDa. Although ubiquitously expressed, the highest levels of Ubc9 are found in testis and thymus. Ubc9 was localized to the synaptonemal complex in male mouse sex chromosomes. Furthermore, Ubc9 interacts with the recombination protein Rad51, thus suggesting an important role for Ubc9 during meiosis.

610748 Rev. 1
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610748 Rev.1
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開発者向け参考資料 (4)

  1. Buschmann T, Lerner D, Lee CG, Ronai Z. The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9. J Biol Chem. 2001; 276(44):40389-40395. (Clone-specific: Western blot). 参考文献を見る
  2. Kovalenko OV, Plug AW, Haaf T. Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes. 1996; 93(7):2958-2963. (Biology). 参考文献を見る
  3. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Clone-specific: Immunofluorescence, Western blot). 参考文献を見る
  4. Wang ZY, Qiu QQ, Seufert W. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996; 271(40):24811-24816. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
610748 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.