Purified Mouse Anti-TFII-I

BD Transduction Laboratories™ Purified Mouse Anti-TFII-I

Name: Purified Mouse Anti-TFII-I
Brand: BD Transduction Laboratories™
SKU: 610942

クローン 42/TFII-I

(RUO)

Western blot analysis of TFII-I on HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-TFII-I.

Immunofluorescent staining of FHS cells.

Western blot analysis of TFII-I on HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-TFII-I.

Immunofluorescent staining of FHS cells.

Spectrum Viewer

Protocol Library

Scientific Resources

製品詳細

Brand: BD Transduction Laboratories™

Alternative Name: BAP-135

Reactivity: Human (QC Testing), Mouse, Rat, Dog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human BAP-135 aa. 17-123

Application: Western blot (Routinely Tested), Immunofluorescence (Tested During Development)

Target Molecular Weight: 135/140 kDa

Concentration: 250 µg/ml

RRID: AB_398255

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

推奨アッセイ手順

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610942 Rev. 1

抗体の詳細

42/TFII-I

General transcription factor II-I (GTF2I/TFII-I/SPIN/BAP-135) is a transcription factor that contains six directly repeated 90-residue regions, which possess helix-loop-helix protein-protein interaction motifs. TFII-I can regulate transcription in T-cells through interaction with the initiator elments (Inrs) within the AdML and Vβ promoters, and associates with HIV-1, TdT, and ribonucleotide reductase R1 Inrs. In addition, TFII-I associates with the E box motif, the CACGTG sequence, and with serum response element sequences. The helix-loop-helix repeats facilitate TFII-I interaction with other transcription factors, such as USF, Myc, Phox 1, MADS box protein serum response factor, and STATs. TFII-I was also identified as Bruton's tyrosine kinase (btk)-associated protein (BAP-135). TFII-I/BAP-135 is tyrosine phosphorylated by btk and after EGF stimulation, and this phosphorylation enhances TFII-I transcriptional activity. The wide expression of TFII-I and the interaction of TFII-I with various Inrs and transcription factors implicates TFII-I in various signaling pathways that regulate gene transcription.

610942 Rev. 1

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

610942 Rev.1

引用＆参考文献

Development References (1)

Yang W, Desiderio S. BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement. Proc Natl Acad Sci U S A. 1997; 94(2):604-609. (Biology). View Reference

610942 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.