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Purified Mouse Anti-SIII p15
Purified Mouse Anti-SIII p15

Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Elongin C/SIII p15 antibody.

Purified Mouse Anti-SIII p15

Immunofluorescent staining of Human Fibroblasts.

Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Elongin C/SIII p15 antibody.

Immunofluorescent staining of Human Fibroblasts.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Frog (Tested in Development)
Mouse IgG1
Human SIII p15 aa. 1-112
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
15 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610761 Rev. 1
抗体の詳細
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56/SIIIp15

SIII p15 is one of three polypeptides that comprise the approximate 140kDa RNA polymerase II transcription factor SIII. The other two polypeptides have the molecular weights of 110kDa and 18kDa. All three polypeptides are required for transcription factor SIII function. Although the polypeptides were identified and purified from rat liver nuclear extracts, tissue expression of this transcription factor appears to be widespread. RNA polymerase II recognizes the SIII transcription factor and in turn initiates transcription from these correctly initiated transcripts. A yeast transcription factor designated YES may be the yeast homolog to SIII.

610761 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610761 Rev.1
引用&参考文献
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開発者向け参考資料 (2)

  1. Bradsher JN, Jackson KW, Conaway RC, Conaway JW. RNA polymerase II transcription factor SIII. I. Identification, purification, and properties. J Biol Chem. 1993; 268(34):25587-25593. (Biology). 参考文献を見る
  2. Kuznetsova AV, Meller J, Schnell PO. von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination. Proc Natl Acad Sci U S A. 2003; 100(5):2706-2711. (Clone-specific: Western blot). 参考文献を見る
610761 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.