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Purified Mouse Anti-Rab3
Purified Mouse Anti-Rab3

Western blot analysis of Rab3 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the anti-Rab3 antibody.

Purified Mouse Anti-Rab3

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Western blot analysis of Rab3 on a rat cerebrum lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the anti-Rab3 antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

製品詳細
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BD Transduction Laboratories™
Rat (QC Testing), Dog (Tested in Development)
Mouse IgG2a
Rat Rab3A aa. 60-220
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
25 kDa
250 µg/ml
AB_397762
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610379 Rev. 1
抗体の詳細
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9/Rab3

The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles, and are important for vesicular traffic. It is thought that Rab proteins cycle through GTP- and GDP-bound forms and that this cycle is related to their function. Four highly homologous (77-85% identity) Rab3 proteins have been described: Rab3A, Rab3B, Rab3C, and Rab3D. Rab3A reportedly is expressed in neuronal and neuroendocrine cells and is associated with synaptic and synaptic-like vesicles. Rab3A is involved at a late stage of neurotransmitter exocytosis, perhaps at the recruitment of vesicles at the presynaptic membrane. Rab3B and Rab3C are also reportedly predominantly expressed in neurons and neuroendocrine tissues. In contrast, Rab3D has been reported to be highly enriched in fat cells and probably does not function sequentially in the same exocytic pathway as Rab3A.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610379 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610379 Rev.1
引用&参考文献
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開発者向け参考資料 (2)

  1. Novick P, Brennwald P. Friends and family: the role of the Rab GTPases in vesicular traffic. Cell. 1993; 75(4):597-601. (Biology). 参考文献を見る
  2. Yi Z, Yokota H, Torii S et al. The Rab27a/Granuphilin Complex Regulates the Exocytosis of Insulin-Containing Dense-Core Granules. Mol Cell Biol. 2002; 22:1858-1867. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). 参考文献を見る
610379 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.