Skip to main content Skip to navigation
Purified Mouse Anti-PRK1
製品詳細
Down Arrow Up Arrow


BD Transduction Laboratories™
PKN
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human PRK1 aa. 215-388
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
120 kDa
250 µg/ml
AB_398011
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610686 Rev. 1
抗体の詳細
Down Arrow Up Arrow
49/PRK1

Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.

610686 Rev. 1
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610686 Rev.1
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (5)

  1. Flynn P, Mellor H, Casamassima A, Parker PJ. Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase. J Biol Chem. 2000; 275(15):11064-11070. (Clone-specific: Western blot). 参考文献を見る
  2. Hughes WE, Larijani B, Parker PJ. Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ. J Biol Chem. 2002; 277(25):22974-22979. (Biology: Western blot). 参考文献を見る
  3. Mukai H, Kitagawa M, Shibata H. Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis. Biochem Biophys Res Commun. 1994; 204(1):348-356. (Biology). 参考文献を見る
  4. Mukai H, Kitagawa M, Shibata H. Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis. Biochem Biophys Res Commun. 1994; 204(1):348-356. (Biology). 参考文献を見る
  5. Palmer RH, Ridden J, Parker PJ. Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family. Eur J Biochem. 1995; 227(1-2):344-351. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610686 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.