Skip to main content Skip to navigation
Purified Mouse Anti-PP5
Purified Mouse Anti-PP5

Western blot analysis of PP5 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-PP5 antibody.

Purified Mouse Anti-PP5

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Western blot analysis of PP5 on a rat cerebrum lysate.  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-PP5 antibody.

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

製品詳細
Down Arrow Up Arrow


BD Transduction Laboratories™
PPT
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Rat PP5 aa. 36-238
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
58 kDa
250 µg/ml
AB_398334
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611020 Rev. 1
抗体の詳細
Down Arrow Up Arrow
3/PP5

Phosphorylation and dephosphorylation on serine and threonine residues is critical for signal transduction and the regulation of numerous cellular functions.  Phosphorylation levels are modulated by protein kinases and phosphatases.  The Ser/Thr phosphatase PP5 (also known as PPT) is a model protein of the fourth subfamily of the PPP-family of phosphatases which includes PP1, PP2A, and PP2B.  PP5 is primarily located in the nucleus, demonstrates widespread tissue distribution, and is highly conserved among species.  It consists of a C-terminal catalytic domain and an N-terminal TPR (tetratricopeptide repeat) domain which is involved in protein-protein interactions.  The TPRs of PP5 mediate protein phosphatase activity.  TPRs mediate the interaction of PP5 with various proteins including the hsp90-glucocorticoid receptor complex and the kinase domain of  ANP-guanylate cyclase receptor.  PP5 is thought to be important in cell growth.  For example, it functions upstream of p53 to regulate p21/WAF1/Cip1-mediated G1 growth arrest.  Thus, PP5 is a nuclear protein phosphatase that is involved in signaling pathways of cell growth and arrest.  

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611020 Rev. 1
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611020 Rev.1
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (5)

  1. Becker W, Kentrup H, Klumpp S, Schultz JE, Joost HG. Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain. J Biol Chem. 1994; 269(36):22586-225920. (Biology). 参考文献を見る
  2. Chen MX, McPartlin AE, Brown L, Chen YH, Barker HM, Cohen PT. A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus. EMBO J. 1994; 13(18):4278-4290. (Biology). 参考文献を見る
  3. Das AK, Cohen PW, Barford D. The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions. EMBO J. 1998; 17(5):1192-1199. (Biology). 参考文献を見る
  4. Ollendorff V, Donoghue DJ. The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J Biol Chem. 1997; 272(51):32011-32018. (Biology). 参考文献を見る
  5. Zuo Z, Dean NM, Honkanen RE. Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21(WAF1/Cip1) and mediate growth arrest. J Biol Chem. 1998; 273(20):12250-12258. (Biology). 参考文献を見る
すべて表示する (5) 表示項目を減らす
611020 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.