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Purified Mouse Anti-Plectin
Purified Mouse Anti-Plectin

Western blot analysis of Plectin on HeLa cells. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Plectin.

Purified Mouse Anti-Plectin

Immunofluorescence staining of HISM

Western blot analysis of Plectin on HeLa cells. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Plectin.

Immunofluorescence staining of HISM

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Plectin aa. 3062-3184
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
400/500 kDa
250 µg/ml
AB_398870
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611348 Rev. 1
抗体の詳細
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31/Plectin

Plectin is an intermediate filament-binding protein that acts as a cross-linking elementin the cytoskeleton. Sequence analysis reveals that plectin is dumbbell shaped and contains a long central α-helical coiled-coil rod flanked by globular domains. Plectinhas six C-terminal repeats that exhibit a tandemly repeated amino acid sequence motif that is characteristic of intermediate filament-associated proteins, such as BPAG1 and desmoplakin. Plectin associates with the sarcolemma, nuclear membrane, and intermyofibrillar network in muscle and with hemidesmosmes in skin. These localizations may be a result of plectin binding to a variety of cytoskeletal proteins including laminin B, microtubule-associated proteins, and α-spectrin. In addition, plectin interacts with G-actin in a PIP2-dependent manner and with actin stress fibers viaits N-terminal actin-binding domain. Patients with epidermolysis bullosa simplex, adisease that results in structural and functional abnormalities in muscle and skin, have no plectin expression in muscle tissues and deficient plectin levels in skin. Thus, plectin cytoskeletal association may be critical for the structural integrity of muscle and skin.

611348 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611348 Rev.1
引用&参考文献
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開発者向け参考資料 (4)

  1. Andra K, Nikolic B, Stocher M, Drenckhahn D, Wiche G. Not just scaffolding: plectin regulates actin dynamics in cultured cells. Genes Dev. 1998; 12(21):3442-3451. (Biology). 参考文献を見る
  2. Banwell BL, Russel J, Fukudome T, Shen XM, Stilling G, Engel AG. Myopathy, myasthenic syndrome, and epidermolysis bullosa simplex due to plectin deficiency. J Neuropathol Exp Neurol. 1999; 58(8):832-846. (Biology). 参考文献を見る
  3. Liu CG, Maercker C, Castanon MJ, Hauptmann R, Wiche G. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 1996; 93(9):4278-4283. (Biology). 参考文献を見る
  4. Wiche G, Krepler R, Artlieb U, Pytela R, Denk H. Occurrence and immunolocalization of plectin in tissues. J Cell Biol. 1983; 97(3):887-901. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
611348 Rev. 1

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