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Purified Mouse Anti-Phosphotyrosine
Purified Mouse Anti-Phosphotyrosine

Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.

Purified Mouse Anti-Phosphotyrosine

Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.

Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

製品詳細
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog, Frog (Tested in Development)
Mouse IgG2a
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
1.0 mg/ml
AB_397806
Aqueous buffered solution containing glycerol and ≤0.09% sodium azide.
RUO


推奨アッセイ手順

The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610430 Rev. 1
抗体の詳細
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PY69

Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.

610430 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610430 Rev.1
引用&参考文献
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開発者向け参考資料 (5)

  1. Doronin S, Shumay E, Wang HY, Malbon CC. Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin. J Biol Chem. 2002; 277(17):15124-15131. (Clone-specific: Western blot). 参考文献を見る
  2. Doronin S, Wang Hy HY, Malbon CC. Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase. J Biol Chem. 2002; 277(12):10698-10703. (Clone-specific: Western blot). 参考文献を見る
  3. Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988; 109(2):277-285. (Biology). 参考文献を見る
  4. Moro L, Dolce L, Cabodi S, et al. Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p13Cas and leads to phosphorylation of specific EGF receptor tyrosines. J Biol Chem. 2002; 277(11):9405-9414. (Clone-specific: Western blot).
  5. Yan Z, Deng X, Friedman E. Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III. J Biol Chem. 2001; 276(2):1555-1563. (Clone-specific: Western blot). 参考文献を見る
すべて表示する (5) 表示項目を減らす
610430 Rev. 1

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