Skip to main content Skip to navigation
Purified Mouse Anti-Human MST1
Purified Mouse Anti-Human MST1

Western blot analysis of MST1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human MST1 antibody.

Purified Mouse Anti-Human MST1

Immunofluorescence staining of human endothelial cells.

Western blot analysis of MST1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human MST1 antibody.

Immunofluorescence staining of human endothelial cells.

製品詳細
Down Arrow Up Arrow


BD Transduction Laboratories™
Mammalian Sterile Twenty-like 1; Mammalian STE20-like kinase-1
Human (QC Testing)
Mouse IgG1
Human MST1 aa. 331-483
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
56 kDa
250 µg/ml
AB_398365
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611052 Rev. 1
抗体の詳細
Down Arrow Up Arrow
7/MST1

Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module.  Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation.  Members of the first class (Ste20, Cla4, and p21-activated protein kinase [PAK]) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42-binding domain.  Members of the second class lack GTPase-binding sites, but are similar to the former class throughout the catalytic domain.  The latter class includes GC kinase, HPK, KHS, KRS1 & 2, MST1, 2, & 3, and SOK-1.  MST1 (Mammalian Sterile Twenty-like-1) is a ubiquitously expressed kinase that contains an N-terminal kinase domain and C-terminal dimerization and inhibitory domains.  Apoptotic stimuli, such as anti-Fas, result in cleavage of the C-terminal regulatory domain by caspase-3 or a related caspase, and activation of MST1.  Overexpression of MST1 induces caspase activity, apoptotic morphological changes, and the activation of the SAPK and p38 MAPK pathways.  Thus, MST1 is thought to function as a component of a positive feedback loop that amplifies the apoptotic response.

611052 Rev. 1
フォーマットの詳細
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611052 Rev.1
引用&参考文献
Down Arrow Up Arrow

開発者向け参考資料 (2)

  1. Creasy CL, Chernoff J. Creasy CL, Chernoff J. J Biol Chem. 1995; 270(37):21695-21700. (Biology). 参考文献を見る
  2. Lian JP, Toker A, Badwey JA. Phosphorylation of the activation loop of gamma p21-activated kinase (gamma-Pak) and related kinases (MSTs) in normal and stressed neutrophils. J Immunol. 2001; 166(10):6349-6357. (Biology: Western blot). 参考文献を見る
611052 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.