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Purified Mouse Anti-Human HS1
Purified Mouse Anti-Human HS1

Western blot analysis of HS1 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HS1 antibody.

Purified Mouse Anti-Human HS1

Immunofluorescent staining of HL60 cells with anti-HS1 antibody.

Western blot analysis of HS1 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HS1 antibody.

Immunofluorescent staining of HL60 cells with anti-HS1 antibody.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human HS1 aa. 17-190
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
75 kDa
250 µg/ml
AB_397898
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610540 Rev. 1
抗体の詳細
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9/HS1

HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610540 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610540 Rev.1
引用&参考文献
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開発者向け参考資料 (3)

  1. Fukuda T, Kitamura D, Taniuchi I. Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate. Proc Natl Acad Sci U S A. 1995; 92(16):7302-7306. (Biology). 参考文献を見る
  2. Takemoto Y, Furuta M, Li XK, Strong-Sparks WJ, Hashimoto Y. LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck. EMBO J. 1995; 14(14):3403-3414. (Biology). 参考文献を見る
  3. Yamanashi Y, Okada M, Semba T. Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling. Proc Natl Acad Sci U S A. 1993; 90(8):3631-3635. (Biology). 参考文献を見る
610540 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.