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Purified Mouse Anti-Human DDX1
Purified Mouse Anti-Human DDX1

Weatern blot analysis of DDX1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human DDX1 antibody.

Purified Mouse Anti-Human DDX1

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Weatern blot analysis of DDX1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human DDX1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human DDX1 aa. 351-544
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
82 kDa
250 µg/ml
AB_398741
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611207 Rev. 1
抗体の詳細
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22/DDX1

DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611207 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611207 Rev.1
引用&参考文献
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開発者向け参考資料 (4)

  1. Amler LC, Schurmann J, Schwab M. The DDX1 gene maps within 400 kbp 5' to MYCN and is frequently coamplified in human neuroblastoma. Genes Chromosomes Cancer. 1996; 15(2):134-137. (Biology). 参考文献を見る
  2. Godbout R, Packer M, Bie W. Overexpression of a DEAD box protein (DDX1) in neuroblastoma and retinoblastoma cell lines. J Biol Chem. 1998; 273(33):21161-21168. (Biology). 参考文献を見る
  3. Grundhoff AT, Kremmer E, Tureci O, et al. Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C. J Biol Chem. 1999; 274(27):19136-19144. (Biology). 参考文献を見る
  4. Squire JA, Thorner PS, Weitzman S, et al. Co-amplification of MYCN and a DEAD box gene (DDX1) in primary neuroblastoma. Oncogene. 1995; 10(7):1417-1422. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす
611207 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.