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Purified Mouse Anti-Heme Oxygenase 1
Purified Mouse Anti-Heme Oxygenase 1

Western blot analysis of Heme Oxygenase 1 on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Heme Oxygenase 1.

Western blot analysis of Heme Oxygenase 1 on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Heme Oxygenase 1.

製品詳細
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Heme Oxygenase 1 (HO-1) aa. 150-286
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
32 kDa
250 µg/ml
AB_398036
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


製品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
抗体の詳細
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23/Heme Oxygenase 1

The 23/Heme Oxygenase 1 monoclonal antibody specifically recognizes Heme oxygenase 1 (HO-1), also known as Heat Shock Protein 32 (HSP32). HO-1 is a 32 kDa monooxygenase encoded by HMOX1. Heme oxygenases 1 and 2 (HO-1, HO-2) cleave the heme molecule, resulting in the production of carbon monoxide (CO) and biliverdin. While HO-2 is constitutively expressed in tissues, HO-1 is rapidly induced by several stimuli such as lutathione depletion, hemin, heat shock, heavy metals, oxidative stress, oxidized LDL, anoxia, and endotoxic shock. HO-1 and its byproducts have potent antioxidant, antiproliferative, anti-inflammatory and anti-apoptotic effects in various cell types. In humans, HO-1 deficiency is associated with susceptibility to oxidative stress, increased inflammation and endothelial cell damage. In immune cells, HO-1 is required for the activation of interferon (IFN) regulatory factor 3 (IRF3) after Toll-like receptor 3 or 4 stimulation or viral infection. It is also upregulated on T cells upon TCR stimulation.

フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
引用&参考文献
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開発者向け参考資料 (4)

  1. Foresti R, Clark JE, Green CJ, Motterlini R. Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells. Involvement of superoxide and peroxynitrite anions. J Biol Chem. 1997; 272(29):18411-18417. (Biology). 参考文献を見る
  2. Ishikawa K, Navab M, Leitinger N, Fogelman AM, Lusis AJ. Induction of heme oxygenase-1 inhibits the monocyte transmigration induced by mildly oxidized LDL. J Clin Invest. 1997; 100(5):1209-1216. (Biology). 参考文献を見る
  3. Suzuki M, Ishizaka N, Tsukamoto K. Pressurization facilitates adenovirus-mediated gene transfer into vein graft. FEBS Lett. 2000; 470(3):370-374. (Clone-specific: Western blot). 参考文献を見る
  4. Yoshida T, Biro P, Cohen T, Muller RM, Shibahara S. Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem. 1988; 171(3):457-461. (Biology). 参考文献を見る
すべて表示する (4) 表示項目を減らす

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